Aclacinomycin A inhibited the in vitro conversion of phage phi X174 single-stranded DNA to the replicative form DNA. DNA synthesis was inhibited by 50% in the presence of 15 microM aclacinomycin A. The inhibition was competitive with respect to template DNA (Ki = 13 microM) and was reversed by addition of Escherichia coli cell extracts. Short complementary strands approximately one-third of unit length molecule were synthesized in the presence of 15 microM aclacinomycin A. The data suggest that aclacinomycin A may inhibit the process of phi X174 DNA chain elongation by a direct interaction with the E. coli host enzymes.
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