Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1432-1033.1978.tb11991.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Construction of a Vero cell line expression human KREMEN1 for the development of CVA6 vaccines.
Virol J
January 2025
State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, 361102, PR China.
Coxsackievirus A6 (CVA6) has emerged as a major pathogen causing hand, foot and mouth disease (HFMD) outbreaks worldwide. The CVA6 epidemic poses a new challenge in HFMD control since there is currently no vaccine available against CVA6 infections. The Vero cell line has been widely used in vaccine production, particularly in the preparation of viral vaccines, including poliovirus vaccines and EV71 vaccines.
View Article and Find Full Text PDFThe role of MEGF10 in myoblast fusion and hypertrophic response to overload of skeletal muscle.
J Muscle Res Cell Motil
January 2025
School of Molecular and Cellular Biology, University of Leeds, Leeds, LS2 9JT, UK.
Biallelic mutations in multiple EGF domain protein 10 (MEGF10) gene cause EMARDD (early myopathy, areflexia, respiratory distress and dysphagia) in humans, a severe recessive myopathy, associated with reduced numbers of PAX7 positive satellite cells. To better understand the role of MEGF10 in satellite cells, we overexpressed human MEGF10 in mouse H-2k-tsA58 myoblasts and found that it inhibited fusion. Addition of purified extracellular domains of human MEGF10, with (ECD) or without (EGF) the N-terminal EMI domain to H-2k-tsA58 myoblasts, showed that the ECD was more effective at reducing myoblast adhesion and fusion by day 7 of differentiation, yet promoted adhesion of myoblasts to non-adhesive surfaces, highlighting the importance of the EMI domain in these behaviours.
View Article and Find Full Text PDFCo-expression of the RPS6KB1 and PDPK1 genes for production of activated p70S6K1 using bac-to-bac baculovirus expression system.
Mol Biol Rep
January 2025
Department of Structural and Molecular Biology, University College London, London, WC1E 6BT, UK.
Background: Ribosomal protein S6 kinase 1 (p70S6K1) is a member of the AGC family of serine/threonine kinases which plays a role in various cellular processes, including protein synthesis, cell growth, and survival. Dysregulation of p70S6K1, characterized by its overexpression and/or hyperactivation, has been implicated in numerous human pathologies, particularly in several types of cancer. Therefore, generating active, recombinant p70S6K1 is critical for investigating its role in cancer biology and for developing novel diagnostic or therapeutic approaches.
View Article and Find Full Text PDF<b>Background and Objectives:</b> This study characterized a bacterial strain, BN122, isolated from the root tissues of purple sticky rice (<i>Oryza sativa</i> L. var. glutinosa).
View Article and Find Full Text PDFOptimization of Fermentation Conditions in Ergothioneine Biosynthesis from Ganoderma resinaceum (Agaricomycetes) and an Evaluation of Their Inhibitory Activity on Xanthine Oxidase.
Int J Med Mushrooms
January 2025
Department of Bioengineering, College of Food Science & Institute of Food Biotechnology, South China Agricultural University, Guangzhou 510640, China; Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou 510640, China.
Ganoderma resinaceum is a traditional mushroom that contains natural products, including ergothioneine (EGT), which has powerful antioxidant properties in the human body. To increase EGT yield from G. resinaceum, the optimal carbon and nitrogen sources in the culture medium were determined as 20 g/L sucrose and 4 g/L NH4Cl, respectively.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!