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Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans.
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Department of Biochemistry and Medical Chemistry, University of Pecs, Medical School, Pecs, Hungary.
Background And Aims: Recently, demands towards identifying various molecules in support of stress detection and potential clinical utilization are dramatically increasing. Moreover, the accuracy with which researchers quantify these informative molecules is now far more improved when compared to the past. As RNA or protein markers are conventionally detected via repeated invasive procedures from blood, it is critical to develop secure technologies to obtain the desired information via less stressful methodologies, such as saliva collection.
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State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, No. 639 Longmian Road, Nanjing, 211198, China.
Background: Cell membrane chromatography (CMC) is a biochromatography with a dual function of recognition and separation, offering a distinct advantage in screening bioactive compounds from Chinese medicines (CMs). Yindan Xinnaotong soft capsule (YD), a CM formulation, has been widely utilized in the treatment of cardiovascular disease. However, a comprehensive mapping of the myocardial protective active compounds remains elusive.
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Key Laboratory of Sports and Physical Fitness of the Ministry of Education, Beijing Sport University, Beijing, China.
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View Article and Find Full Text PDFMechanism Analysis of Bubble Discharge Within Silicone Gels Under Pulsed Electric Field.
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School of Electrical Engineering, Shandong University, Jingshi Road 17923, Jinan 250061, China.
Silicone gel, used in the packaging of high-voltage, high-power semiconductor devices, generates bubbles during the packaging process, which accelerates the degradation of its insulation properties. This paper establishes a testing platform for electrical treeing in silicone gel under pulsed electric fields, investigating the effect of pulse voltage amplitude on bubble development and studying the initiation and growth of electrical treeing in a silicone gel with different pulse edge times. The relationship between bubbles and electrical treeing in silicone gel materials is discussed.
View Article and Find Full Text PDFApplication two-dimensional gel electrophoresis coupled with LC-MS/MS to identify candidate serodiagnostic antigens for early detection Psoroptes ovis var. cuniculi infection.
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December 2024
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address:
Currently, the 'gold standard' for diagnosis of Psoroptes ovis infections is detecting Psoroptes mites or eggs in skin scrapings under microscopy, but it is prone to be mis-diagnosed for detecting early infection of P. ovis. Hence, seeking a reliable diagnostic technique for detecting early-stage mite infections is extremely desirable.
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