In the absence of depolarizing agents, 45Ca uptake by synaptosomes of the brain tissue of spontaneously hypertensive rats (SHR) was 40% greater than in normotensive controls. This difference disappeared after membrane treatment with depolarizing agents or with the addition of the Ca channel blocker verapamil. Increased 45Ca uptake was also observed in isolated platelets of SHR. It can be assumed that in both cases these differences were caused by partial depolarization of the plasma membrane as a result of its increased permeability to Na+. The addition of calmodulin to microsomal fractions of SHR brain tissue resulted in a considerably diminished increase in ATP-dependent calcium accumulation. These data are in accord with previous findings obtained in a study on the erythrocyte membrane Ca pump. Data obtained by means of differential scanning microcalorimetry of erythrocyte membranes and gel electrophoresis of membrane proteins indicated a higher band-3 protein content in the erythrocyte membranes of SHR. These data support the concept of primary hypertension as a type of generalized membrane pathology.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/00005344-198400061-00006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Deep learning-based design and experimental validation of a medicine-like human antibody library.
Brief Bioinform
November 2024
Biotherapeutics Molecule Discovery, Boehringer Ingelheim Pharmaceutical Inc., 900 Ridgebury Road, Ridgefield, CT 06877, United States.
Antibody generation requires the use of one or more time-consuming methods, namely animal immunization, and in vitro display technologies. However, the recent availability of large amounts of antibody sequence and structural data in the public domain along with the advent of generative deep learning algorithms raises the possibility of computationally generating novel antibody sequences with desirable developability attributes. Here, we describe a deep learning model for computationally generating libraries of highly human antibody variable regions whose intrinsic physicochemical properties resemble those of the variable regions of the marketed antibody-based biotherapeutics (medicine-likeness).
View Article and Find Full Text PDFMeasuring Speech Discrimination Ability in Sleeping Infants Using fNIRS-A Proof of Principle.
Trends Hear
January 2025
Bionics Institute, East Melbourne, VIC, Australia.
This study used functional near-infrared spectroscopy (fNIRS) to measure aspects of the speech discrimination ability of sleeping infants. We examined the morphology of the fNIRS response to three different speech contrasts, namely "Tea/Ba," "Bee/Ba," and "Ga/Ba." Sixteen infants aged between 3 and 13 months old were included in this study and their fNIRS data were recorded during natural sleep.
View Article and Find Full Text PDFEffects of CO and liquid digestate concentrations on the growth performance and biomass composition of and microalgal strains.
Front Bioeng Biotechnol
January 2025
APESA Pôle valorisation, Montardon, France.
This study evaluated the growth performance of and microalgae cultivated in diluted liquid digestate supplemented with CO, comparing their efficiency to that of a conventional synthetic media. The presence of an initial concentration of ammonium of 125 mg N-NH .L combined with the continuous injection of 1% v/v CO enhanced the optimal growth responses and bioremediation potential for both strains in 200-mL cultures.
View Article and Find Full Text PDFIntegrated SegFlow, µSIA, and UPLC for Online Sialic Acid Quantitation of Glycoproteins Directly from Bioreactors.
Eng Life Sci
January 2025
Analytical Development & Analytical Attribute Science in Biologics Bristol Myers Squibb Devens Massachusetts USA.
This study emphasizes the critical importance of closely monitoring and controlling the sialic acid content in therapeutic glycoproteins, including EPO, interferon-γ, Orencia, Enbrel, and others, as the level of sialylation directly impacts their pharmacokinetics (PK), immunogenicity, potency, and overall clinical performance due to its influence on protein clearance via hepatic asialoglycoprotein receptors (ASGPR). The ASGPR recognizes and binds to glycoproteins exposed to terminal galactose or N-acetylgalactosamine residues, leading to receptor-mediated endocytosis. Recent studies have demonstrated that sialylation of O-linked glycan plays a role in protecting against macrophage galactose lectin (MGL)-mediated clearance.
View Article and Find Full Text PDFClimate change and recurrent droughts challenge wheat production and yield, necessitating careful selection and plant breeding research. "Value for Cultivation and Use" experiments are crucial for assessing genetic gains and providing information about potential pathways to alleviate production losses under specific environmental conditions. The goal of the study was to compare the grain yield and quality characteristics of 46 registered bread wheat cultivars in 5 out of 7 agro-ecological regions of Türkiye between 2016-2017 and 2017-2018.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!