We describe a method for covalent binding of insulin to the outer surface of multilamellar liposomes loaded with spin label. Encapsulation of the label Tempocholine-nitroxide within the aqueous phases of liposomes is controlled by Electron Spin Resonance. The binding of insulin is performed using the Carlsson's heterobifunctional reagent: N-succinimidyl 3-(2-pyridyldithio) propionate. The coupling method results in efficient attachment of 2. 64.10(-4) mole of insulin per mole of phospholipid; the integrity of these vesicles is not modified as confirmed by spin resonance analysis. Moreover, the liposome-coupled insulin retains its antigenic specificity as shown by radioimmunoassays.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0006-291x(83)91214-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
HOMA-IR value in predicting retinal microvascular dysfunction.
Narra J
December 2024
Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
Obesity and retinal microvasculature dysfunction are linked and impact visual acuity. The aim of this study was to determine the relationship between the HOMA-IR score and the presence of vascular dysfunction (capillary perfusion and flux index) of the optic nerve head (ONH) of the retina in obese patients and to determine its diagnostic performance to predict vascular dysfunction. A case-control study was conducted in 2022 involving individuals from obese and non-obese groups.
View Article and Find Full Text PDF: A Potential Target for the Prevention of Diabetes.
Foods
December 2024
College of Food Science, Shenyang Agricultural University, Shenyang 110866, China.
, a Gram-negative anaerobic bacterium colonizing the intestinal mucus layer, is regarded as a promising "next-generation probiotic". There is mounting evidence that diabetes and its complications are associated with disorders of abundance. Thus, and its components, including the outer membrane protein Amuc_1100, -derived extracellular vesicles (AmEVs), and the secreted proteins P9 and Amuc_1409, are systematically summarized with respect to mechanisms of action in diabetes mellitus.
View Article and Find Full Text PDFClinical effect of acupuncture on knee osteoarthritis and its effect on p38 MAPK signaling pathway.
Turk J Phys Med Rehabil
September 2024
Department of Needle Knife, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
Objectives: This study aims to investigate the curative efficacy of acupuncture on knee osteoarthritis (KOA) and its improvement on related scores and blood indexes.
Patients And Methods: Between January 2019 and January 2020, a total of 108 patients (48 males, 60 females; mean age: 61.0±6.
Chicken GLUT4 function via enhancing mitochondrial oxidative phosphorylation and inhibiting ribosome pathway in skeletal muscle satellite cells.
Poult Sci
December 2024
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450046, China. Electronic address:
Article Synopsis
- GLUT4 is a key protein in glucose uptake and metabolism, showing significant expression in chicken skeletal muscles and varying across developmental stages of cells.
- Chicken GLUT4 expression increases in response to insulin treatment, enhancing cellular processes like proliferation and glucose consumption but later inhibiting glucose uptake with high glucose levels.
- Overexpression of GLUT4 leads to the identification of 302 differentially expressed genes linked to metabolic pathways, with notable changes in oxidative phosphorylation and ribosomal processes.
Identification of Sinapic Acid Derivatives from Petit Vert Leaves and Their Effects on Glucose Uptake in C2C12 Murine Myoblasts.
Biomolecules
October 2024
Shizuoka Shin-Food Development Corp., Shizuoka 422-8064, Japan.
Petit vert (scientific name: var. DC. × var.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!