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Differential inhibition by acetazolamide on carbonic anhydrase distribution in the quail kidney: a proposal for a membrane-bound isoenzyme.
Histochem J
January 1992
Department of Molecular, Cellular and Animal Biology, University of Camerino, Italy.
The effects of different concentrations of acetazolamide, a specific carbonic anhydrase inhibitor, have been investigated in the quail kidney. The histochemical patterns, interpreted by means of quantitative analyses proved that 0.1 microM acetazolamide inhibited the enzyme activity in all the reactive tubular segments except for distal tubules.
View Article and Find Full Text PDFThe quantification of the histochemical protein staining with 2-hydroxy-1-naphthaldehyde (HNA) demonstrating primary amino groups of proteins.
Acta Histochem
July 1991
Institute of Biochemistry, Karl-Franzens-University, Graz, Austria.
The usefulness of the HNA-pH4-1d staining, which histochemically demonstrates primary protein amino groups under the conditions used, for the microphotometric quantification of proteins was investigated. A correlation (r = 0.986) has been found between the mean protein contents of fresh frozen and fixed sections prepared from different tissues of rats and the corresponding mean integrated extinction values determined histophotometrically after HNA-pH4-1d staining.
View Article and Find Full Text PDFRenal carbonic anhydrase in the quail Coturnix coturnix japonica: I. Activity and distribution in male and female metanephros.
Histochem J
November 1990
Department of Cellular Biology, University of Camerino, Italy.
Carbonic anhydrase activity was studied in the quail metanephros by means of histochemical, histophotometrical and biochemical methods. Male and female samples were examined separately in order to show sex-related differences in enzyme activity and localization. The staining patterns revealed differential distribution of reaction product in the different tubular segments.
View Article and Find Full Text PDFFresh frozen and fixed serial sections were stained with 2,2'-dihydroxy-6,6'-dinaphthyldisulfide (DDD) and Fast blue B for reactive protein thiols (PSHr) and total reactive protein sulfur (TRPS). The mean optical densities of PSHr and TRPS determined histophotometrically at a distinct part of a tissue were related to each other (PSHr: TRPS). If this quotient has been determined, e.
View Article and Find Full Text PDFHistophotometric quantification of the field effect and the extended field effect of tumors.
Free Radic Res Commun
January 1990
Institut für Biochemie, Karl-Franzens-Universität Graz, Austria.
Histophotometric investigations have been made on samples of human skin. Fresh frozen serial sections were fixed and stained for either reactive protein thiols (PSHr) or total reactive protein sulphur (TRPS) using modifications of the DDD-Fast blue B-method. In addition, total protein thiols (PSHt) were stained with the Mercurochromcyanide-method, and proteins were stained using a modified amido-black procedure.
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