The ability of the nitroso derivative of the drug cimetidine to interact with cellular macromolecules in the intact rat was investigated. Radio-labelled nitrosocimetidine (NC) was shown to methylate DNA in a variety of tissues in the rat after oral administration. Radioactivity was also detected in the RNA and protein extracted from these same tissues. Methylation of DNA by the parent compound, cimetidine, was not detected in any of the tissues studied. For comparison, the DNA methylation produced by the carcinogen N-methyl-N-nitro-N-nitrosoguanidine (MNNG) dosed orally was measured. DNA alkylation by MNNG was found to be approx. 2-36 times greater than that produced by NC, varying with the tissues studied. The highest yield of DNA alkylation was found in the stomach for MNNG and the small intestine for nitrosocimetidine suggesting pharmacokinetic differences.
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http://dx.doi.org/10.1016/0009-2797(82)90097-7 | DOI Listing |
Gland Surg
December 2024
Department of Gynecology, Wenzhou People's Hospital, Wenzhou, China.
Background: Infertility is a special reproductive health defect. For women, congenital uterine malformations, extensive adhesions in the uterine cavity, and hysterectomy are associated with infertility. Uterine transplantation is technically feasible, but its clinical application and development are limited by donor shortages and immune rejection.
View Article and Find Full Text PDFJ Nucl Med
January 2025
Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts;
Spinal cord injuries (SCIs) often lead to lifelong disability. Among the various types of injuries, incomplete and discomplete injuries, where some axons remain intact, offer potential for recovery. However, demyelination of these spared axons can worsen disability.
View Article and Find Full Text PDFArch Biochem Biophys
January 2025
Institute of Cell Biophysics RAS - a separate subdivision of Federal Research Centre "Pushchino Scientific Centre for Biological Research RAS", Institutskaya St., 3, 142290, Russia, Moscow Region, Pushchino. Electronic address:
The possibility of using an oxygen-nitrous oxide mixture for prolonged hypothermic preservation of rat heart for 24 hours was investigated. A comparative analysis of restoration of functional activity of hearts in the groups of 24-hour preservation at +4°C with different gases (O, N) and gas mixtures (CO+O, NO+O, N+O, NO+N) was carried out. It was shown that the presence of oxygen in the gas mixture was the key factor for heart preservation.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Department of Physiological Sciences, Interinstitutional Post-Graduate Program of Physiological Sciences, Federal University of São Carlos (UFSCar), São Carlos 13.566-490, SP, Brazil.
Menopause occurs due to the depletion of the ovarian reserve, leading to a progressive decline in estrogen (E2) levels. This decrease in E2 levels increases the risk of developing several diseases and can coexist with chronic kidney disease (CKD). Arterial hypertension (AH) is another condition associated with menopause and may either contribute to or result from CKD.
View Article and Find Full Text PDFCells
January 2025
Institute of Anatomy & Cell Biology, Faculty of Medicine, Justus-Liebig-University, Aulweg 123, 35392 Giessen, Germany.
Vascular smooth muscle cell (SMC) relaxation by guanylyl cyclases (GCs) and cGMP is mediated by NO and its receptor soluble GC (sGC) or natriuretic peptides (NPs) ANP/BNP and CNP with the receptors GC-A and GC-B, respectively. It is commonly accepted that cultured SMCs differ from those in intact vessels. Nevertheless, cell culture often remains the first step for signaling investigations and drug testing.
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