Glycoproteins from Trypanosoma cruzi epimastigotes have been extracted by diiodosalycilic acid and lithium salts, and phenol-water biphasic partition. Peanut agglutinin has been used in a one step preparative method for fractionating the total extract in order to separate the so-called galactose-terminal glycoproteins. The different fractions have been studied by SDS electrophoresis, ultracentrifugation and immunoelectrophoresis techniques. The experimental immunogenicity, antigenicity and specificity of the PNA affinity fractions has been evaluated.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1365-3024.1982.tb00424.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of L-carnitine and pentoxifylline on long-term preservation of the human sperms: An experimental study.
Int J Reprod Biomed
November 2024
Histomorphometry and Stereology Research Center, Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Background: In infertility clinics, long-time preserving high-quality spermatozoa is a challenging problem.
Objective: The present study aimed to prolong preserving of the human spermatozoa by adding pentoxifylline (PT) and L-carnitine (LC) without using high-cost freezing techniques.
Materials And Methods: In this experimental study, semen samples of 26 normozoospermia men aged between 28-34 yr, were firstly prepared using the swim-up technique, and each sample was divided into the following 3 aliquots: untreated control group, the LC, and PT-treated groups.
Heterogeneity in Fluorescence-Stained Sperm Membrane Patterns and Their Dynamic Changes Towards Fertilization in Mice.
Front Biosci (Landmark Ed)
January 2025
Graduate School of Life and Environmental Sciences, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, 400-8510 Kofu, Japan.
Background: Sperm represent a heterogeneous population crucial for male reproductive success. Additionally, sperm undergo dynamic changes during maturation and capacitation. Despite these well-established processes, the complex nature of sperm heterogeneity and membrane dynamics remains elusive.
View Article and Find Full Text PDFAntibacterial and anticancer activities of three novel lectin-conjugated chitosan nanoparticles.
Appl Microbiol Biotechnol
November 2024
Department of Botany, Faculty of Science, Ain Shams University, Cairo, Egypt.
To the best of our knowledge, this is the first attempt to synthesize, characterize, and determine the antibacterial and anticancer effects of three novel conjugates of plant lectins: phytohemagglutinin lectin (PHA), soybean agglutinin (SBA), and peanut agglutinin (PNA) with chitosan nanoparticles (CHNPs). The lectin concentration within prepared conjugates was estimated using nannodrop, and the highest concentration was 0.96 mg/ml in PHA-CHNPs.
View Article and Find Full Text PDFLectin histochemistry in the small intestines of piglets naturally infected with porcine epidemic diarrhea virus.
J Vet Sci
September 2024
College of Veterinary Medicine and Host-Directed Antiviral Research Center, Chonnam National University, Gwangju 61186, Korea.
Importance: Porcine epidemic diarrhea virus (PEDV) binds to particular cell surface receptors to penetrate cells. The virus specifically identifies certain carbohydrate structures present on the surface of the cell to facilitate the binding process. Nevertheless, the influence of viral infections on specific alterations of glycoconjugates in the small intestines remains unexplored.
View Article and Find Full Text PDFCyclodextrin-Cholesterol Alone or in Combination With Cyclodextrin-Vitamin E Improves Bull Sperm Cryopreservation in a Soybean Lecithin Extender.
Reprod Domest Anim
October 2024
Department of Veterinary Sciences, Laboratoire Environnement, Santé et Production Animale (LESPA), Institute of Veterinary Sciences and Agronomic Sciences, University of Batna 1, Batna, Algeria.
Combining cholesterol-loaded methyl-β-cyclodextrin (CD-CHL) with vitamin E-loaded methyl-β-cyclodextrin (CD-Vit E) to combat cold shock and oxidative stress during sperm cryopreservation in soybean lecithin extenders remains unexplored. Thus, the current study aimed to investigate the effect of treating bull sperm with CD-CHL and CD-Vit E prior to cryopreservation in a soybean lecithin extender. Sperm collected from eight fertile bulls were pooled and split into six aliquots.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!