The polycation DEAE-dextran treatment of HeLa cells was found to interfere with the production of reovirus, appearance of viral cytopathology, and the induction of cytotoxicity by UV-irradiated reovirus. The data obtained showed that while the polycation pre-treatment of cells enhanced virus adsorption to cells, its addition early during virus adsorption or 2 h after infection markedly interfered with virus production. The interference was decreasingly effective when the polycation was added during the later stages of the infectious cycle. Isopycnic CsCl buoyant density ultracentrifugation analyses of infected cytoplasmic extracts revealed that there were more subviral particles formed in untreated infected cells than there were in polycation-treated cells. In vitro uncoating studies with infected cytoplasmic extracts indicated that the polycation did not interfere with the removal of the outer capsid structure of the complete virus, and uncoating occurred only in the presence of the polycation. Electron microscopical examination revealed significantly fewer virus particles present in polycation-treated infected cells. The accumulated data strongly indicate that interference by DEAE-dextran is an early event involving viral penetration. Corollary studies using other polycations, such as polybrene (hexadimethrine bromide and poly-l-lysine, revealed that while they also enhanced virus adsorption, they did not interfere with reovirus production, appearance of viral cytopathology, and the induction of cytotoxicity by UV-irradiated reovirus.
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Viruses
January 2025
Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, MN 55108, USA.
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January 2025
Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, Yerevan 0014, Armenia.
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January 2025
State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, 430062, Wuhan, China; Hubei Jiangxia Laboratory, 430200, Wuhan, China. Electronic address:
Pseudorabies virus (Pseudorabiesvirus, PRV) has caused huge economic losses to the global pig industry. In recent years, it has been reported that there are PRV mutants, but the traditional vaccine can not completely prevent or control the infection of PRV, so there is an urgent need to develop new broad-spectrum anti-disease drugs for prevention and treatment. PNGase F from bacteria can catalyze the hydrolysis of oligosaccharides linked to asparagine residues on peptides, so we speculate that PNGase F can inhibit virus infection by removing the glycosylation of virus membrane glycoproteins.
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January 2025
Department of Bioengineering, University of California Riverside, Riverside, CA, 92521, USA. Electronic address:
African Swine Fever Virus (ASFV) is a highly contagious pathogen with nearly 100% mortality in swine, causing severe global economic loss. Current detection methods rely on nucleic acid amplification, which requires specialized equipment and skilled operators, limiting accessibility in resource-constrained settings. To address these challenges, we developed the Covalently Immobilized Magnetic Nanoparticles Enhanced CRISPR (CIMNE-CRISPR) system.
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