A rapid one-step gradient centrifugation method to prepare PMN leucocytes has been worked out by which a 98--99% pure, washed granulocyte suspension of 98% viability can be obtained in 30--40 minutes. The cells prepared by this method displayed higher NBT reduction upon ingesting the same soluble DNA-anti DNA complexes than those prepared by the dextran sedimentation method.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
centrifugation method
8
rapid centrifugation
4
method
4
method isolation
4
isolation polymorphonuclear
4
polymorphonuclear leucocytes
4
leucocytes human
4
human blood
4
blood rapid
4
rapid one-step
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!