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Dynamic changes of heterogeneous cell wall macromolecules in differentiating conifer xylem using cytochemical localization.
Int J Biol Macromol
January 2025
Department of Wood Anatomy and Utilization, Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China; Wood Specimen Resource Center (WOODPEDIA) of National Forestry and Grassland Administration, Beijing 100091, China. Electronic address:
Article Synopsis
- Understanding the changes in cell wall components during xylem differentiation is crucial for grasping the structure of wood, especially at the secondary cell wall layer level.
- In the study of Pinus bungeana, it was found that crystalline cellulose and glucomannan were deposited before xylan and lignin, with lignification starting in the primary cell wall corners as the S layer developed.
- Immunofluorescence results showed that glucomannan was deposited earlier than xylan, and while pectins were concentrated in the primary walls, they decreased as tracheid walls differentiated, with ray cells lagging behind axial tracheids in their development.
Cytochemical localization and synthesis mechanism of the glucomannan in pseudobulbs of Reichb. f.
Hortic Res
May 2024
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
The dried pseudobulbs of , an important traditional Chinese medicine named , have an extraordinary polysaccharide content and excellent prospects for medicinal effects. However, the distribution and molecular mechanism underlying biosynthesis are poorly understood. In this study, chemical and immunologic analyses were performed in representative tissues of , and the results showed that what are conventionally termed polysaccharides (BSPs) are water-soluble polysaccharides deposited only in pseudobulbs.
View Article and Find Full Text PDF[1, 25-(OH)-VitD3 attenuates renal tubulointerstitial fibrosis in diabetic kidney disease by inhibiting Snail1-SMAD3/SMAD4 complex formation].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
April 2023
Department of Nephrology, Guizhou Provincial People's Hospital; NHC Key Laboratory of Pulmonary Immunological Disease, Guiyang 550002, China. *Corresponding author, E-mail:
Objective To investigate the effect of 1, 25-(OH)-VitD3 (VitD3) on renal tubuleinterstitial fibrosis in diabetic kidney disease. Methods NRK-52E renal tubular epithelial cells were divided into control group (5.5 mmol/L glucose medium treatment), high glucose group (25 mmol/L glucose medium treatment) and high glucose with added VitD3 group (25 mmol/L glucose medium combined with 10 mmol/L VitD3).
View Article and Find Full Text PDFReactivity of selected markers of innate and adaptive immunity in rabbits experimentally infected with antigenic variants of RHD (Lagovirus europaeus/GI.1a).
Vet Res Commun
February 2022
Institute of Veterinary Sciences, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100, Toruń, Poland.
Lagovirus europaeus/GI.1 causes a fatal viral condition in rabbits characterized by acute viral hepatitis and disseminated intravascular coagulation. Due to rapid viral and environmental changes variants (Lagovirus europaeus/GI.
View Article and Find Full Text PDFClinical Significance of MicroRNA-29a and MicroRNA-100 Gene Expression in Pediatric Acute Myeloid Leukemia.
J Pediatr Hematol Oncol
March 2022
Pediatric Oncology, National Cancer Institute, Cairo University.
Aim: The aim of this study was to evaluate the diagnostic and prognostic performance of miRNA-29a and miRNA-100 in pediatric acute myeloid leukemia (AML).
Patients And Methods: In all, 73 children with diagnosed pediatric AML (based on standard morphologic, cytochemical, cytogenetic, immunologic, and molecular workup, and the French-American British classification) admitted to Children's Cancer Hospital Egypt (CCHE-57357), and 9 healthy age-matched and sex-matched controls were recruited for a case-control study. Gene expression levels of miRNA-29a and miRNA-100 were assessed using real-time quantitative RT-PCR.
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