The effect of various modes of tissue preparation on the retention of distinct serum proteins was determined. Specific and sensitive radioimmunologic methods were used to measure serum alphafetoprotein (AFP) and albumin(SA) in 105,000 x g hypotonic soluble (cytoplasm) and hypertonic (400mM KCl) solubilized pellets (nuclear extracts) from homogenates of immature rat estrogen target tissues. The tissue level of both serum antigens was influenced by the preparative method: cervical dislocation followed by organ isolation and a single rinse yielded the highest levels of apparent tissue AFP and SA while decapitation followed by exsanguination, organ isolation and multiple rinses resulted in the lowest levels. For the uterus, exposure of the uterine lumen enhanced reduction of serum antigen detection. The recommended procedure minimizes the inclusion of serum proteins which can interfere in the subsequent in vitro measurement of specific receptors in hormone responsive tissues.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/01971528008055788 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Diagnostic Performance of PIVKA-II in Italian Patients with Hepatocellular Carcinoma.
Cancers (Basel)
January 2025
Department of Medical and Surgical, Maternal-Infantile and Adult Sciences, University of Modena and Reggio Emilia, 41126 Modena, Italy.
Background And Aims: Hepatocellular carcinoma (HCC) represents the second leading cause of cancer deaths worldwide. Six-month imaging along with alpha-fetoprotein (AFP) serum levels detection are the current gold standard to exclude HCC. Protein induced by vitamin K absence (PIVKA-II) has been proposed as a potential screening biomarker for HCC.
View Article and Find Full Text PDFNon-contrast magnetic resonance imaging versus ultrasonography for hepatocellular carcinoma surveillance: A randomized, single-center trial.
Gastroenterology
January 2025
Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea.
Background & Aims: This study aimed to compare ultrasonography (US) and non-contrast magnetic resonance imaging (MRI) in the surveillance of hepatic malignancy.
Methods: We conducted a randomized, non-blinded, single-center trial at a single center in South Korea. Eligible individuals were aged 20-70 years with liver cirrhosis, Child-Pugh class A, and no history of liver cancer or other recent malignancy.
Highly Sensitive Digital and Analog Immunoassay Based on Submicron Magnetic Beads and Fluorescent Microspheres.
Anal Chem
January 2025
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
The detection of disease-related protein biomarkers plays a crucial role in the early diagnosis, treatment, and monitoring of diseases. The concentrations of protein biomarkers can vary significantly in different diseases or stages of the same disease. However, most of the existing analytical methods cannot simultaneously meet the requirements of high sensitivity and a wide dynamic range.
View Article and Find Full Text PDFAntidiabetic Effects of Quercetin and Silk Sericin in Attenuating Dysregulation of Hepatic Gluconeogenesis in Diabetic Rats Through Potential Modulation of PI3K/Akt/FOXO1 Signaling: In Vivo and In Silico Studies.
J Xenobiot
January 2025
Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), Alexandria 21934, Egypt.
Type 2 diabetes mellitus (T2DM) is an intricate disease correlated with many metabolic deregulations, including disordered glucose metabolism, oxidative stress, inflammation, and cellular apoptosis due to hepatic gluconeogenesis aberrations. However, there is no radical therapy to inhibit hepatic gluconeogenesis disturbances yet. We thus sought to probe the effectiveness and uncover the potential mechanism of quercetin (QCT) and silk sericin (SS) in mitigating hyperglycemia-induced hepatic gluconeogenesis disorder, which remains obscure.
View Article and Find Full Text PDFHepatoid adenocarcinoma of the stomach with ideal response to neoadjuvant chemo-immunotherapy: a case report.
Front Immunol
January 2025
Department of General Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong, China.
Hepatoid adenocarcinoma of the stomach (HAS) is a rare subtype of gastric cancer characterized by histological features resembling hepatocellular carcinoma. Surgical intervention remains the preferred treatment modality for eligible patients. However, the efficacy of neoadjuvant therapy and alternative treatment regimens has been found to be suboptimal.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!