AI Article Synopsis
- A cDNA for human leukocyte interferon was created and inserted into the pBR322 vector, then cloned in E. coli.
- The resulting DNA sequence includes a 23-amino acid signal peptide followed by a 165-amino acid interferon polypeptide.
- An expression plasmid was developed that allows E. coli to produce a large amount of interferon, which successfully protected squirrel monkeys from a serious viral infection.
Article Abstract
A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid signal peptide followed by an interferon polypeptide of 165 amino acids. An expression plasmid was constructed which permits the synthesis in E. coli of 2.5 x 10(8) units of interferon per litre of culture. This LeIF protected squirrel monkeys from lethal encephalomyocarditis virus infection.
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| http://dx.doi.org/10.1038/287411a0 | DOI Listing |
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