HPLC procedures with electrochemical or ultraviolet detection were developed in order to analyze plasma samples from patients and volunteers who had received antipsychotic drugs. Procedures were described for the determination of chlorpromazine and trimeprazine in human plasma. The described procedures for chlorpromazine and trimeprazine demonstrated sufficient sensitivity for their use in pharmacokinetic studies following low single oral doses of these agents. The HPLC-EC procedures for chlorpromazine and trimeprazine were compared with GC-MS and radioimmunoassay procedures, respectively.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0278-5846(83)90049-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Role of Phenothiazines and Structurally Similar Compounds of Plant Origin in the Fight against Infections by Drug Resistant Bacteria.
Antibiotics (Basel)
February 2013
Department of Medical Microbiology and Immunology, University of Szeged, Dom ter 10, H-6720, Szeged, Hungary.
Phenothiazines have their primary effects on the plasma membranes of prokaryotes and eukaryotes. Among the components of the prokaryotic plasma membrane affected are efflux pumps, their energy sources and energy providing enzymes, such as ATPase, and genes that regulate and code for the permeability aspect of a bacterium. The response of multidrug and extensively drug resistant tuberculosis to phenothiazines shows an alternative therapy for the treatment of these dreaded diseases, which are claiming more and more lives every year throughout the world.
View Article and Find Full Text PDFQuantitative determination of phenothiazine derivatives in human plasma using monolithic silica solid-phase extraction tips and gas chromatography-mass spectrometry.
J Chromatogr A
May 2011
Department of Legal Medicine, Showa University School of Medicine, Tokyo, Japan.
Solid-phase extraction (SPE) using micropipette tips is a useful technique to prepare samples prior to mass spectrometry. However, most commercial SPE tips have loading capacities that are insufficient for quantitative determination. In this paper, we describe a rapid method for quantitative microanalysis of five phenothiazine derivatives, chlorpromazine, levomepromazine, promazine, promethazine and trimeprazine, using a recently introduced C(18) monolithic silica SPE tip, the MonoTip C(18), for extraction from human plasma.
View Article and Find Full Text PDFSimultaneous determination of some phenothiazine derivatives in human blood by headspace solid-phase microextraction and gas chromatography with nitrogen-phosphorus detection.
J AOAC Int
March 2009
Showa University School of Medicine, Department of Legal Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
Chlorpromazine, levomepromazine, promazine, triflupromazine, and trimeprazine were simultaneously determined in human whole blood and plasma by combining headspace solid-phase microextraction and gas chromatography with nitrogen-phosphorus detection. Extraction efficiency for the phenothiazine derivatives was 0.013-0.
View Article and Find Full Text PDFInactivation of cholinesterase induced by chlorpromazine cation radicals.
Pharmacol Toxicol
February 2003
Department of Biology, Hokkaido College of Pharmacy, Katuraoka-cho 7-1, Otaru 047-0264, Japan.
To clarify the mechanism of the side effect of chlorpromazine, we examined the inactivation of cholinesterase induced by chlorpromazine. Cholinesterase was inactivated and its activity was lost in rat serum during interaction of chlorpromazine with horseradish peroxidase and H2O2. When chlorpromazine was oxidized by horseradish peroxidase and H2O2, the reaction solution colored pink and the visible absorption spectrum was consistent with the absorption spectrum of the chlorpromazine cation radical (CPZ*+).
View Article and Find Full Text PDFPhenothiazine radicals inactivate Trypanosoma cruzi dihydrolipoamide dehydrogenase: enzyme protection by radical scavengers.
Free Radic Res
March 2003
Bioenergetics Research Centre, School of Medicine, University of Buenos Aires, Paraguay 2155, 1121-Bueno Aires, Argentina.
Phenothiazine cation radicals (PTZ+*) irreversibly inactivated Trypanosoma cruzi dihydrolipoamide dehydrogenase (LADH). These radicals were obtained by phenothiazine (PTZ) peroxidation with myeloperoxidase (MPO) or horseradish peroxidase (HRP/H2O2) systems. LADH inactivation depended on PTZ structure and incubation time.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!