In rat liver microsomes freezing with subsequent thawing led to irreversible redistribution of protein-lipid packing. This redistribution was detected by a change in the efficiency of energy transfer between protein aromatic groups of membrane protein and lipid-soluble fluorescent probe pyrene. Transglutaminase pretreatment of microsomes prevented the irreversible redistribution. The enzyme is shown to bind no more than 15 per cent of the whole membrane protein. This smaller part of the microsomal protein is supposed to play the decisive role in the movements of its remaining part.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Cascading tipping points of Antarctica and the Southern Ocean.
Ambio
December 2024
College of Business and Economics, University of Tasmania, Hobart, Australia.
Antarctica and the Southern Ocean are key elements in the physical and biological Earth system. Human-induced climate change, and other human activities in the region, are leading to several potential interacting tipping points with major and irreversible consequences. Here, we examine eight potential physical, biological, chemical, and social Antarctic tipping points.
View Article and Find Full Text PDFLinear and Two-Dimensional Infrared Spectroscopy of the Multifunctional Vibrational Probe, 3-(4-Azidophenyl) Propiolonitrile. Deperturbing a Fermi Triad by Isotopic Substitution.
Chemphyschem
December 2024
Clausius-Institut für Physikalische und Theoretische Chemie, Rheinische Friedrich-Wilhelms-Universität Bonn, Wegelerstrasse 12, 53115, Bonn.
Infrared probes are chemical moieties whose vibrational modes are used to obtain spectroscopic information about structural dynamics of complex systems; in particular, of biomacromolecules. Here, we explore the vibrational spectroscopy and dynamics of a reagent, 3-(4-azidophenyl)propiolonitrile (AzPPN), for selectively tagging thiols in protein environments with a multifunctional infrared probe containing both, an azide and a nitrile chromophore. The linear infrared spectrum of AzPPN is heavily perturbed in the antisymmetric azide stretching region as a result of accidental Fermi resonances.
View Article and Find Full Text PDFCrosstalk between adipogenesis and aging: role of polyphenols in combating adipogenic-associated aging.
Immun Ageing
November 2024
Department of Physiology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
In the last forty years, the number of people over 60 years of age has increased significantly owing to better nutrition and lower rates of infectious diseases in developing countries. Aging significantly impacts adipose tissue, which plays crucial role in hormone regulation and energy storage. This can lead to imbalances in glucose, and overall energy homeostasis within the body.
View Article and Find Full Text PDFHollow Calcium/Copper Bimetallic Amplifier for Cuproptosis/Paraptosis/Apoptosis Cancer Therapy via Cascade Reinforcement of Endoplasmic Reticulum Stress and Mitochondrial Dysfunction.
ACS Nano
October 2024
State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
The endoplasmic reticulum (ER) and mitochondria are essential organelles that play crucial roles in maintaining cellular homeostasis. The simultaneous induction of ER stress and mitochondrial dysfunction represents a promising yet challenging strategy for cancer treatment. Herein, a hollow calcium-copper bimetallic nanoplatform is developed as a cascade amplifier to reinforce ER stress and mitochondrial dysfunction for breast cancer treatment.
View Article and Find Full Text PDFVisualization of Blood-Brain Barrier Disruption in Septic Mice with the New Method Based on in Vivo Imaging Technology.
Neurocrit Care
December 2024
Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
Background: Dynamic monitoring of the blood-brain barrier (BBB) functional status in septic mice can help to explore the pathological mechanisms. Therefore, we proposed a new method for monitoring BBB permeability and applied it to the detection of sepsis models.
Methods: The new method involves the construction of an optical cranial window and in vivo imaging.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!