Nucleic-acid sandwich hybridisation detected adenovirus in nasopharyngeal aspirates from children with acute respiratory infections within 20 h. The differentiation between subgroups of adenovirus (B and C) and the lack of false positives indicated the specificity of the method. The reference test was detection of an adenovirus protein (hexon) by means of radioimmunoassay. In nucleic-acid sandwich hybridisation the sample DNA mediates the binding of a labelled "probe" DNA fragment to a second DNA fragment bound on filter. The sample DNA itself is not fixed to a solid support, and the only pretreatment required is boiling in a detergent solution. The results indicate that the sandwich hybridisation is specific, quantitative, easy to do, and only marginally affected by the crude nature of the sample. It may therefore be a valuable addition to the range of rapid methods in microbial diagnosis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0140-6736(83)91500-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Experimental Study on Mechanical Performance of Single-Side Bonded Carbon Fibre-Reinforced Plywood for Wood-Based Structures.
Materials (Basel)
January 2025
Department of Integrated Design and Tribology Systems, Faculty of Mechanics and Technology, Rzeszów University of Technology, ul. Kwiatkowskiego 4, 37-450 Stalowa Wola, Poland.
In addition to the traditional uses of plywood, such as furniture and construction, it is also widely used in areas that benefit from its special combination of strength and lightness, particularly as a construction material for the production of finishing elements of campervans and yachts. In light of the current need to reduce emissions of climate-damaging gases such as CO, the use of lightweight construction materials is very important. In recent years, hybrid structures made of carbon fibre-reinforced plastics (CFRPs) and metals have attracted much attention in many industries.
View Article and Find Full Text PDFEffect of Depth of Cut and Number of Layers on the Surface Roughness and Surface Homogeneity After Milling of Al/CFRP Stacks.
Materials (Basel)
January 2025
Faculty of Mechanical Engineering, Poznan University of Technology, Pl. Marii Skłodowskiej-Curie 5, 60-965 Poznań, Poland.
A multilayer structure is a type of construction consisting of outer layers and a core, which is mainly characterized by high strength and specific stiffness, as well as the ability to dampen vibration and sound. This structure combines the high strength of traditional materials (mainly metals) and composites. Currently, sandwich structures in any configurations (types of core) are one of the main directions of technology development and research.
View Article and Find Full Text PDFPrussian Blue Analogues "Dressed" in MXene Nanosheets Tightly for High Performance Lithium-Ion Batteries.
Adv Mater
January 2025
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu, 225002, P. R. China.
MXenes, have been considered as a new generation anode material in lithium-ion batteries for lower lithium-ion diffusion barriers and superior conductivity. Unfortunately, their structures are prone to aggregation and stacking, hindering further shuttle of lithium ions and electrons, resulting in lower discharge capacity. Therefore, the introduction of interlayer spacers for the preparation of MXene-based hybrids has attracted much attention.
View Article and Find Full Text PDFDevelopment of a sandwich-type electrochemical DNA sensor based on CeO/AuPt nanoprobes for highly sensitive detection of hepatitis B virus DNA.
Bioelectrochemistry
January 2025
The Key Laboratory of Biomedical Material, School of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003 China. Electronic address:
To provide accurate diagnostic evidence for early hepatitis B virus (HBV) infection-related diseases, this study targeted HBV DNA as an analyte, where a sandwich-type electrochemical DNA sensor based on gold nanoparticles/reduced graphene oxide (Au NPs/ERGO) and cerium oxide/gold-platinum nanoparticles (CeO/AuPt NPs) was constructed. Au NPs/ERGO composite nanomaterials were first synthesized on the surface of a glass carbon electrode using electrochemical co-reduction, which significantly improved the specific surface area and electrical conductivity of the electrode. Further specific hybridization of target HBV-DNA was performed by combining capture probe DNA (S1-DNA) bound to AuNPs/ERGO with CeO/AuPt modified signal probe DNA (S2-DNA).
View Article and Find Full Text PDFCrystal structure of -poly[bis-(,-di-methyl-hydroxyl-ammonium) [di-μ-bromido-di-bromido-stannate(II)]].
Acta Crystallogr E Crystallogr Commun
January 2025
Department of Chemistry, Taras Shevchenko National University of Kyiv, Volodymyrska str. 64/13, 01601 Kyiv, Ukraine.
The title compound, {(CHNO)[SnBr]} , is a layered hybrid perovskite crystallizing in the monoclinic space group 2/. The asymmetric unit consists of one HC-O-NH -CH cation (MeHA), one Sn atom located on a twofold rotation axis, and two Br atoms. The Sn atom has a distorted octa-hedral coordination environment formed by the bromido ligands.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!