Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Comparison of Paired Immunofluorescent Antibody Serology and Real-Time Polymerase Chain Reaction Testing for the Detection of Acute Q Fever among Febrile Patients in Kilimanjaro, Tanzania, 2012-2014.
Am J Trop Med Hyg
December 2024
Division of Infectious Diseases and International Health, Department of Medicine, Duke University, Durham, North Carolina.
Acute Q fever diagnosis via paired serology is problematic because it requires follow-up for convalescent sample collection; as such, it cannot provide a diagnosis to inform a treatment decision at the time of acute presentation. Real-time polymerase chain reaction (PCR) may be a useful approach for the diagnosis of acute Q fever in endemic settings. Among febrile patients enrolled in a sentinel surveillance study for Q fever at two referral hospitals in Moshi, Tanzania, from 2012 to 2014, we analyzed those with paired sera for IgG to Coxiella burnetii (C.
View Article and Find Full Text PDFComputational studies on metabolic pathways of Coxiella burnetii to combat Q fever: A roadmap to vaccine development.
Microb Pathog
January 2025
Department of Animal Sciences, School of Life Sciences, Central University of Himachal Pradesh, District Kangra, Himachal Pradesh, India, 176206. Electronic address:
Coxiella burnetii (Cbu) is the gram-negative intracellular pathogen responsible for deadly zoonotic infection, Q fever. The pathogen is environmentally stable and distributed throughout the world which is sustained in nature by chronic infection of ruminants. The epidemiological studies on Q fever indicates it as emerging public health problem in various countries and it is imperative to promptly identify an appropriate therapeutic solution for this pathogen.
View Article and Find Full Text PDFMolecular mechanisms of formalin-fixed cellular vaccine reactogenicity.
Infect Immun
November 2024
Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
Article Synopsis
- The Q-VAX vaccine has not been licensed outside Australia due to local and systemic reactogenic responses in previously sensitized individuals, which are not well understood at the cellular level.
- A mouse model study revealed that localized reactions at the vaccination site involve CD8+ and IL17a+ CD4+ T cells, and both antibodies and CD4+ T cells are crucial for these localized responses.
- The study found that whole cell vaccine material can persist at the injection site for up to 26 weeks, with more severe reactions linked to higher levels of small cell variants in the vaccine formulation, supporting the idea that prolonged antigen presence contributes to reactogenicity.
Local and systemic reactogenic responses to Q-VAX® have prevented licensing of this vaccine outside of Australia. These reactogenic responses occur in previously sensitize individuals and have not been well defined at the cellular level, in part because many studies have been done in guinea pigs that have limited molecular tools. We previously characterized a mouse model of reactogenicity where local reactions sites showed an influx of CD8+ and IFNγ-expressing IL17a+ CD4+ T cells consistent with a Th1 delayed-type hypersensitivity.
View Article and Find Full Text PDFEfficiency of recombinant Ybgf in a double antigen-ELISA for the detection of Coxiella antibodies in ruminants.
Vet Anim Sci
September 2024
Department of Veterinary Medicine and Animal Productions, University of Naples, "Federico II", Naples 80137, Italy.
Q fever is a zoonosis whose main reservoirs are domestic ruminants. Surveillance in these species is carried out mainly with serological tests, which, however, have limited diagnostic performance, and their manufacturing requires laboratories equipped with high biosafety requirements for antigen production. Recombinant ELISAs do not depend on these requirements and, being based on a single antigen, can reduce potential false positivity by identifying antibodies specific to a phase of infection.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!