An interference microscopic method is described which allows quantitative measurements of the inhibiting effect of the di-sodium salt of 1,3-bis(3-carboxy-chroman-5-yl-oxy)-2-hydroxypropand (cromoglicinic acid; Intal; DSCG) on the degranulation of mast cells in vitro induced by polymixin B and Compound 48/80. The specific degranulation inhibiting effect of DSCG is increased in the presence of Ca++ and Mg++ ions.
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