The influence of iron at different concentrations on the growth and toxin formation of the cultures of C. perfringens strain 28 BP6K, type A, in media having different composition and protein base has been studied. As revealed by the results of these studies, bivalent iron at concentrations of 1-30 mg%, while having no essential influence on the growth and development of the culture, not only produces no stimulating effect on toxin formation, but even inhibits it. The concentrations of iron, both producing the optimum effect on toxin formation and inhibiting it, are not identical for different kinds of culture media and depend on their nature and composition. In culture media with fodder yeast extract added, and especially in media based on fermentative fodder yeast hydrolysate, the cultures have shown the increased stability of toxin formation in the presence of higher concentrations of iron.

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