Total casein phosphatase activity of erythrocytes from one-month-old rats was separated by DEAE-cellulose chromatography into three peaks--E1, E2 and E3--and only into two peaks--E1 and E3--when the erythrocyte donors were six- and 12-month-old rats. The activity of E1 (Mr 330 K) decreased continuously in erythrocytes during the first year of postnatal life. E2 (Mr 230 K) also decreased and completely disappeared from the cells of 12-month-old rats. E3 (Mr 180 K) was the dominant molecular form in the cytosol of erythrocytes during the first year of life. It decreased only up to six months of life. In this form E3 seems to be cooperative with respect to the substrate and to inhibitor molecules. The decrease of its kinetic parameters (Vmax and K0.55) was also found during postnatal ontogenesis. E3 isolated from erythrocytes of older rats (6 and 12 months) was more susceptible to inhibitory effect of pyrophosphate and to the change of ionic strength of eluting buffer than the enzyme from one-month-old rats. 0.2 mol.1(-1) NaCl lowered Mr of E3 phosphatase from 180 K to 128 K only in older rats.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0300-9084(84)90087-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exposure to Di-n-butyl Phthalate Causes Modulation in Neurotransmitter System of Wistar Rats: A Multigenerational Assessment.
Int J Toxicol
December 2024
Department of Zoology, Bangalore University, Bangalore, India.
Neuroendocrine regulation is disrupted by di-n-butyl phthalate (DBP) when exposure occurs during the critical periods of fetal development, which can lead to neurological disorders. To evaluate the toxic potential of DBP, it is necessary to conduct teratological studies, which could determine impacts on the development of the fetus. The present study was designed to understand the sequelae of neuroendocrine regulation in one-month-old pups when rats were exposed to DBP (F-F) and during lactation.
View Article and Find Full Text PDFStudy on the expression of testin in the testes of dogs.
J Vet Res
December 2023
Department of Veterinary Medicine and Animal Science, University of Milan, 26900 Lodi, Italy.
Introduction: Testin is a protein involved in cell mobility, adhesion and colony formation. In rats, testin presence has been reported in the testes, and its possible role in spermatogenesis has been suggested. Studies in humans also suggest a possible role of testin as a cancer suppressor protein.
View Article and Find Full Text PDFA STUDY OF THE INFLUENCE OF JUVENILE ADJUVANT ARTHRITIS ON DENTAL HARD TISSUES CONDITION IN EXPERIMENTAL ANIMALS.
Wiad Lek
December 2023
NATIONAL PIROGOV MEMORIAL MEDICAL UNIVERSITY, VINNYTSIA, UKRAINE.
Article Synopsis
- The study investigates how juvenile adjuvant-induced arthritis (JAA) affects dental health in rats, focusing on carious tooth lesions.
- Experimental rats showed a significant increase in the number and depth of carious lesions compared to intact rats, with JAA leading to 100% prevalence of dental caries among them.
- The findings highlight the detrimental impact of autoimmune diseases like JAA on dental health, indicating a shift toward more severe carious cavities in affected rats.
Objective: The aim: To analyze the mRNA gene expression level of Aire, Deaf1, Foxp3, Ctla4, Il10, Nlrp3 and distribution of NLRP3+-cells in mesenteric lymph nodes (MLNs) of the offspring of rats with GD, both untreated and treated with glibenclamide and in conditions of insulin oral tolerance formation.
Patients And Methods: Materials and methods: The study involves 160 male rats, one- or six-month-old. The mRNA genes expression was studied by real time quantitative poly¬merase chain reaction.
Rat age-related benign prostate hyperplasia is concomitant with an increase in the secretion of low ramified α-glycosydic polysaccharides.
Theriogenology
September 2022
Dept. Animal Medicine and Surgery, Autonomous University of Barcelona, E-08193, Bellaterra, Cerdanyola del Vallès, Spain. Electronic address:
This work analysed the expression of prostate polysaccharides in rats with age-related benign prostatic hyperplasia (BPH) for a better understanding of the possible relationship between prostate polysaccharides secretion and BPH onset. For this, prostatic glands from 1 month-old, 3 months-old, 6 months-old and 12 months-old Sprague-Dawley rats were processed in order to identify their overall polysaccharide content. Additionally, serum testosterone was also determined.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!