We have derived rat cell lines producing different and regulatable amounts of pp60v-src by introducing the src gene of Rous sarcoma virus (RSV) under the control of the glucocorticoid-responsive transcriptional promoter from the mouse mammary tumor virus (MMTV). We find that the cellular phenotype is strictly dependent upon the dose of pp60v-src with a distinct threshold for changes indicative of neoplastic potential. Cells with low constitutive levels of pp60v-src are not phenotypically distinguishable from cells without v-src, but as little as a 4-fold increment in pp60v-src produces morphological transformation and anchorage-independent growth. These properties of the transformed state are achieved at levels of pp60v-src far below levels found in an RSV-transformed cell line, without detectable increase in phosphorylation of the major cellular target for tyrosine phosphorylation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0092-8674(84)90271-x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dynamic G-Quadruplexes in the Rous Sarcoma Virus Genome: Scaffolds for Protein Interaction and Potential Anti-Viral Targets.
Chembiochem
January 2025
Bose Institute - Centenary Campus, Biophysics, P-1/12 CIT Scheme VIIM, Kankurgachi, Centenary Campus, 700054, KOLKATA, INDIA.
The Rous sarcoma virus (RSV) is an onco-retrovirus that infects avian species such as the chicken (Gallus gallus). RSV is the first oncovirus to be described, and the oncogenic activity of this virus is related to the expression of a tyrosine kinase that induces carcinogenic transformation. Interestingly, we have noted that the RSV genome contains various potential G4-forming sequences.
View Article and Find Full Text PDFRetroviruses are responsible for significant pathology in humans and animals, including the acquired immunodeficiency syndrome and a wide range of malignancies. A crucial yet poorly understood step in the replication cycle is the recognition and selection of unspliced viral RNA (USvRNA) by the retroviral Gag protein, which binds to the psi (Ψ) packaging sequence in the 5' leader, to package it as genomic RNA (gRNA) into nascent virions. It was previously thought that Gag initially bound gRNA in the cytoplasm.
View Article and Find Full Text PDFMammalian ZAP and KHNYN independently restrict CpG-enriched avian viruses.
bioRxiv
December 2024
Department of Microbiology and Immunology, University of Minnesota - Twin Cities, Minneapolis, MN, USA, 55455.
Zoonotic viruses are an omnipresent threat to global health. Influenza A virus (IAV) transmits between birds, livestock, and humans. Proviral host factors involved in the cross-species interface are well known.
View Article and Find Full Text PDFThe histone H3.3 K27M mutation suppresses Ser31phosphorylation and mitotic fidelity, which can directly drive gliomagenesis.
Curr Biol
January 2025
The Hormel Institute, University of Minnesota, Austin, MN 55912, USA; Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address:
Serine 31 is a phospho-site unique to the histone H3.3 variant; mitotic phospho-Ser31 is restricted to pericentromeric heterochromatin, and disruption of phospho-Ser31 results in chromosome segregation defects and loss of p53-dependant G cell-cycle arrest. Ser31 is proximal to the H3.
View Article and Find Full Text PDFA Novel Rat Model to Simulate Positive Margins in the Wound Bed of a Resected Sarcoma.
Plast Reconstr Surg Glob Open
November 2024
From the Division of Plastic and Reconstructive Surgery, Department of Surgery, Stony Brook University Hospital and Medical Center, Stony Brook, NY.
Background: One reason for local recurrence is the presence of positive surgical margins after tumor resection. An animal model accurately representing the microtumor burden will improve our understanding of these surgical margins. Using a rat model, we report a new methodology for creating microscopic tumors.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!