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Use of the Indian muntjac idiogram to align conserved chromosomal segments in sheep and human genomes by chromosome painting.
Genomics
November 1997
Pathology Department, University of Cambridge, United Kingdom.
We have hybridized all 28 chromosome-specific painting probes from the domestic sheep (Ovis aries, 2n = 54) onto metaphase chromosomes of the Indian muntjac deer (Muntiacus muntjak vaginalis, 2n = 6,7) and identified 35 conserved chromosomal segments. Results from this study show that most of the sheep acrocentric chromosomes hybridized to single regions in the Indian muntjac genome. This conserved hybridization pattern supports the concept that the large Indian muntjac chromosomes were derived from multiple tandem fusions from an ancestral deer species.
View Article and Find Full Text PDFResolving ambiguities in the karyotype of domestic sheep (Ovis aries). II. G-, Q-, and R-banded idiograms, and chromosome-specific molecular markers.
Chromosoma
July 1996
AgResearch Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand.
Internally consistent G-, Q- and R-banded karyotypes and idiograms for sheep chromosomes at the 422-band level of resolution are presented. These were derived by sequential Q- to G-staining, and sequential Q- to R-staining of prometaphase spreads prepared from sheep with normal and Robertsonian chromosomes. The fused chromosomes served as stable morphological markers.
View Article and Find Full Text PDFG- and R-banding comparison of sheep (Ovis aries L.) chromosomes.
Cytogenet Cell Genet
December 1994
National Research Council (CNR), I.A.B.B.A.M., Naples, Italy.
G- and R-banded chromosomes of 14 Gentile di Puglia sheep (Ovis aries L., 2n = 54) from southern Italy were compared at the 450-band level (ISCNDA, 1989). GTG-, GBG-, RBG-, and RBA-banding techniques were employed on slides obtained from blood cell cultures that had incorporated BrdU either early or late in the replication cycle.
View Article and Find Full Text PDFHigh-resolution G-banded karyotype and idiogram of the goat: a sheep-goat G-banded comparison.
J Hered
May 1989
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan.
An elongated G-banded karyotype and idiogram of the domestic goat (Capra hircus) is presented. Sheep (Ovis aries)-goat comparison suggests the terminal light band (Xq 2 9) present in the goat is absent in the sheep.
View Article and Find Full Text PDFA Giemsa banding method was used to obtain preparations from which a G-band idiogram for the chromosomes of the Australian Swamp Buffalo (Bubalus bubalis) was constructed. Comparison with the G-banding patterns for goat, sheep, and ox chromosomes showed a remarkably close similarity individual paris, banding pattern homologies for the buffalo metacentric autosomes being identifiable among the acrocentric autosomes of the other species. However, the goat and sheep lacked a comparable autosome to the buffalo 10, the buffalo lacked an autosome comparable to the ox 12, the acrocentric X chromosome of the buffalo banded most closely to the goat X and was least like the ox.
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