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Evaluation of two MALDI-TOF MS systems and extraction methods for identification of filamentous fungi recovered from clinical specimens.
J Clin Microbiol
January 2025
Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, Missouri, USA.
Unlabelled: Rapid and accurate identification of cultured molds is important to determine clinical significance and therapeutic decision-making. Conventional mold identification uses phenotypic macroscopic and microscopic characterization; however, this can take days or weeks for colony maturity and definitive microscopic structure formation, be limited to genus-level identification, and be misidentified due to morphologic mimics or similarities between closely related species. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) revolutionized bacterial and yeast identification but remains uncommon for molds in part because of limited reference libraries.
View Article and Find Full Text PDFCharacterization of fungal carbonyl sulfide hydrolase belonging to clade D β-carbonic anhydrase.
FEBS Lett
January 2025
Department of Symbiotic Science of Environment and Natural Resources, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Carbonyl sulfide hydrolase (COSase) is a unique enzyme that exhibits high activity towards carbonyl sulfide (COS) but low carbonic anhydrase (CA) activity, despite belonging to the CA family. COSase was initially identified in a sulfur-oxidizing bacterium and later discovered in the ascomycete Trichoderma harzianum strain THIF08. The COSase from T.
View Article and Find Full Text PDFComparative studies on modeling and optimization of fermentation process conditions for fungal asparaginase production using artificial intelligence and machine learning techniques.
Prep Biochem Biotechnol
January 2025
Department of Biotechnology, Arunai Engineering College, Tiruvannamalai, India.
The L-asparaginase is commercial enzyme used as chemotherapeutic agent in cancer treatment and food processing agent in backed and fried food industries. In the present research work, the artificial intelligence and machine learning techniques were employed for modeling and optimization of fermentation process conditions for enhanced production of L-asparaginase by submerged fermentation of . The experimental L-asparaginase activity obtained using central composite experiment design was used for optimization.
View Article and Find Full Text PDFDistribution characteristics and analysis of fungal aerosol concentration and particle size in air-conditioned wards in Wuhan, China.
Ann Agric Environ Med
December 2024
School of Biomedical Engineering and Imaging, Hubei University of Science and Technology, Hubei, China.
Article Synopsis
- Fungal contamination in hospital air can impact the health of staff, patients, and caregivers, with a study in Wuhan revealing that factors like temperature, humidity, and seasonal changes significantly influence fungal concentration rather than disease type or personnel density.
- The analysis showed that airborne fungal particle sizes are normally distributed, with the highest proportions found in specific size ranges, but the median diameter remained below 3.19 μm across different departments in both winter and summer.
- The findings suggest a need for improved filtration efficiency for specific fungal particle sizes and the use of appropriate antifungal treatments and hygiene practices in hospital air management.
Clinical Efficacy of Nasoalveolar Moulding Using Aligner NAM During Presurgical Infant Orthopaedics (PSIO) in Infants With Unilateral Cleft Lip and Palate (UCLP): A Retrospective Study.
Orthod Craniofac Res
December 2024
Department of Orthodontics & Dentofacial Orthopaedics, Manav Rachna Dental College, Faridabad, India.
Objective: The study compares and evaluates planned virtual outcomes with actual post-treatment outcomes to assess the accuracy and predictability of clinical results during presurgical infant orthopaedics (PSIO) with AlignerNAM in infants with unilateral cleft lip and palate.
Setting: Institutional study.
Participants: 14 UCLP patients.
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