Sedimentation and diffusion constants were determined for electrophoretically purified Vi antigen before and after mild alkaline hydrolysis. The molecular weight of the intact antigen was found to be 1.7 x 10(6). Mild alkaline hydrolysis completely removed O-acetyl, reduced the molecular weight to 1.0 x 10(5), and lowered the intrinsic viscosity from 8.0 deciliters/g to approximately 0.5 deciliter/g. Infrared spectra of the two antigen preparations were also compared.
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| http://dx.doi.org/10.1128/jb.94.5.1406-1410.1967 | DOI Listing |
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