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Two complementing selection systems based on cca-trimming exonucleases as a tool to monitor, select and evaluate enzymatic features of tRNA nucleotidyltransferases.
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Institute for Biochemistry, Leipzig University, Leipzig, Germany.
tRNA nucleotidyltransferase represents a ubiquitous and essential activity that adds the indispensable CCA triplet to the 3'-end of tRNAs. To fulfill this function, the enzyme contains a set of highly conserved motifs whose coordinated interplay is crucial for the sequence-specific CCA polymerization. In the human enzyme, alterations within these regions have been shown to lead to the manifestation of disease.
View Article and Find Full Text PDFLight energy-driven carbonic anhydrase mediate CO sequestration system with variable-temperature adaptability.
Int J Biol Macromol
January 2025
College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China; Institute of Biomass & Functional Materials, Shaanxi University of Science & Technology, Xi'an 710021, China. Electronic address:
The escalating atmospheric CO₂ concentration urgently demands ecologically friendly mitigation strategies. Compared to alternative catalysts, carbonic anhydrase (CA) demonstrates exceptionally high catalytic efficiency in CO₂ hydration reactions. Nevertheless, traditional CA immobilization techniques exhibit peak enzymatic activity exclusively at optimal temperatures, consequently constraining their effective application across diverse environmental thermal conditions in industrial settings.
View Article and Find Full Text PDFInvestigating the impact of Multiwalled Carbon Nanotubes exposure on enzymatic activities and histopathological variations in Swiss albino mice.
Sci Rep
January 2025
Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia.
Present study was conducted to evaluate the detrimental impacts of exposure of Multi-walled Carbon Nanotubes (MWCNT-NP) on enzymatic activities and tissue structures in Swiss albino mice. The experimental groups of mice received MWCNT-NP for specific time period (seven or fourteen days). Two distinct doses of the MWCNT-NP solution were given orally: 0.
View Article and Find Full Text PDFA comprehensive study of liver-gut microbiota and antioxidant enzyme activity mediated regulation of late-laying hens by high and low residual feed intake.
Int J Biol Macromol
January 2025
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. Electronic address:
Residual feed intake (RFI) is a better indicator of feed efficiency than feed conversion ratio (FCR). It is frequently used to evaluate the efficacy of poultry and livestock feed consumption. Generally, Low RFI (LRFI) is associated with better feed conversion efficiency, whereas high RFI (HRFI) suggests poorer feed conversion efficiency.
View Article and Find Full Text PDFApplication of a near-infrared viscosity-responsive fluorescent probe for lysosomal targeting in fatty liver mice.
Bioorg Chem
January 2025
Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province, College of Pharmacy, Qinghai Minzu University, Xining 810007 Qinghai, China; State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China. Electronic address:
Viscosity is a fundamental property in biological systems, influencing organelle function and molecular diffusion. Abnormal viscosity is associated with diseases such as metabolic disorders, neurodegeneration, and cancer. Lysosomes, central to cellular degradation and recycling, are sensitive to viscosity changes, which can disrupt enzymatic activity and cellular homeostasis.
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