AI Article Synopsis
- Rabbit globin cDNA synthesized by AMV reverse transcriptase has a small hairpin structure at its 3' end.
- This cDNA can serve as a template for E. coli DNA polymerase I to create a complementary strand.
- Sequential treatments with reverse transcriptase, DNA polymerase I, and S1 nuclease lead to the production of complete double-stranded synthetic globin genes.
Article Abstract
Full-length, single-stranded rabbit globin cDNA, synthesized by AMV reverse transcriptase, apparently contains a small double-stranded sequence (hairpin) at the 3' terminus. This cDNA can serve as template-primer for E. coli DNA polymerase I, which synthesizes a strand complementary to the cDNA and covalently bound to it. The loop connecting the two strands can be cut by S1 nuclease. Reassociation, hybridization, and restriction endonuclease studies, as well as electrophoretic analyses, indicate that the sequential actions of reverse transcriptase, DNA polymerase 1, and S1 nuclease generate full-length, double-stranded synthetic globin genes.
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| http://dx.doi.org/10.1016/0092-8674(76)90027-1 | DOI Listing |
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