Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Identification of f.a., sp. nov. and large indels in the rRNA cistron that split the genus.
Int J Syst Evol Microbiol
January 2025
National Fungal Culture Collection of India (NFCCI), Biodiversity and Palaeobiology Group, MACS-Agharkar Research Institute, Pune, India.
Yeast strains representing a novel asexual ascomycetous species were isolated from seven flowers. Sequencing of the chromosomal regions coding for the D1/D2 domains of the large subunit ribosomal RNA, the ITS1-5.8S-ITS2 segments and parts of the gene coding for the small subunit ribosomal RNA showed that the isolates were conspecific.
View Article and Find Full Text PDFDeveloping Topics.
Alzheimers Dement
December 2024
University of California, San Francisco, San Francisco, CA, USA.
Background: Mitochondrial DNA copy number (mtDNAcn) quantifies the number of mitochondria genomes per nucleated cell, with reduced mtDNAcn being associated with increased Alzheimer's disease (AD) neuropathology. Blood-based mtDNAcn has technical confounders, such as DNA purification, and biological confounders, such as compensatory upregulation of mtDNA. Therefore, we optimized a protocol for mtDNAcn quantification using droplet digital PCR (ddPCR) by testing (i) whole peripheral blood mononuclear cells (PBMCs) vs platelet-depleted PBMCs, (ii) column-based DNA extraction vs cell lysate, and (iii) mitochondrial DNA replication (mtDNArep).
View Article and Find Full Text PDFvClean: assessing virus sequence contamination in viral genomes.
NAR Genom Bioinform
March 2025
Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan.
Recent advancements in viral metagenomics and single-virus genomics have improved our ability to obtain the draft genomes of environmental viruses. However, these methods can introduce virus sequence contaminations into viral genomes when short, fragmented partial sequences are present in the assembled contigs. These contaminations can lead to incorrect analyses; however, practical detection tools are lacking.
View Article and Find Full Text PDFUnlocking Therapeutic Potential: Mesenchymal Stem Cells-derived Exosomes in IUA Treatment, Current Status and Perspectives.
Curr Pharm Des
January 2025
Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Intrauterine adhesion (IUA) is a condition caused by damage to the basal uterine layer which can lead to partial or full occlusion of the uterine cavity. Although traditional treatment options have been useful in mild and moderate cases, they have been unsatisfactory in severe IUA cases. Therefore, it is essential to improve the treatment strategies of IUA.
View Article and Find Full Text PDFApplication of aqueous two-phase extraction for separation and purification of various adeno-associated viruses.
Biotechnol Lett
January 2025
Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang Key Laboratory of Smart Biomaterials, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China.
Objective: Adeno-associated viruses (AAVs) are widely used as gene therapy vectors due to their safety, stability, and long-term expression characteristics. The objective of this work is to develop an aqueous two-phase system (ATPS) as a universal platform for the separation and purification of AAVs.
Results: This study utilized polyethylene glycol (PEG)/salt ATPSs to separate and purify various AAV serotypes, including AAV5, AAV8, and AAV9, which focusing on serotype-specific performance and partial empty capsid removal.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!