A direct method is proposed to select less toxic mutants of Aspergillus terricola on a culture of fibroblasts. The conidia are first irradiated with UV, and then are used to grow colonies on the glass surface of tubes or flasks containing medium 199. The cells of fibroblasts are added thereupon and the two cultures are being grown together for 24--48 hours. The colonies which inhibit the growth of fibroblasts to a less extent are selected using microscopy. The method can be used for primarily selection of experimentally produced mutants of Aspergillus which form fibrinolytic enzymes possessing low toxicity.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Characterization of the complete mitochondrial genome of (Aspergillaceae, Eurotiales), isolated from soy sauce fermentation system.
Mitochondrial DNA B Resour
December 2021
School of China Alcoholic Drinks, Luzhou Vocational and Technical College, Sichuan, Luzhou, P. R. China.
In the present study, the complete mitochondrial genome of É.J. Marchal 1893 was sequenced and assembled.
View Article and Find Full Text PDFControl of by Indigenous Antagonistic Yeast from Vineyards.
Foods
December 2020
Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Avd. Adolfo Suárez s/n, 06007 Badajoz, Spain.
Biocontrol is one of the most promising alternatives to synthetic fungicides for food preservation. , and section are the most concerning pathogens for grape development. However, frequently, other species, such as in this study, are predominant in spoiled bunches.
View Article and Find Full Text PDFDiversity and spoilage potential of microbial communities associated with grape sour rot in eastern coastal areas of China.
PeerJ
June 2020
Shandong Academy of Grape, Jinan, China.
As a polymicrobial disease, sour rot decreases grape berry yield and wine quality. The diversity of microbial communities in sour rot-affected grapes depends on the cultivation site, but the microbes responsible for this disease in eastern coastal China, has not been reported. To identify the microbes that cause sour grape rot in this important grape-producing region, the diversity and abundance of bacteria and fungi were assessed by metagenomic analysis and cultivation-dependent techniques.
View Article and Find Full Text PDFDNA Stable-Isotope Probing Delineates Carbon Flows from Rice Residues into Soil Microbial Communities Depending on Fertilization.
Appl Environ Microbiol
March 2020
Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, China
Decomposition of crop residues in soil is mediated by microorganisms whose activities vary with fertilization. The complexity of active microorganisms and their interactions utilizing residues is impossible to disentangle without isotope applications. Thus, C-labeled rice residues were employed, and DNA stable-isotope probing (DNA-SIP) combined with high-throughput sequencing was applied to identify microbes active in assimilating residue carbon (C).
View Article and Find Full Text PDFFungal Planet description sheets: 868-950.
Persoonia
June 2019
Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD Utrecht, The Netherlands.
Novel species of fungi described in this study include those from various countries as follows: , and on leaves, , and on leaves, and on leaves, on leaves, on litter of regenerating subtropical rainforest, (incl. gen. nov.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!