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alpha-MSH decreases apoptosis in ischaemic acute renal failure in rats: possible mechanism of this beneficial effect.
Nephrol Dial Transplant
August 2001
Department of Internal Medicine, Korea University Hospital, Seoul, Korea.
Background: Apoptosis frequently occurs in acute renal injury but the molecular mechanisms responsible for this distinct form of cell death are largely unknown. Fas belongs to the tumour necrosis factor (TNF)/nerve growth factor superfamily and engagement by Fas ligand induces apoptosis in various epithelial cells. To investigate the role of apoptosis and associated mechanisms, we examined the occurrence of apoptosis and Fas and Fas ligand expression, and the therapeutic effect of alpha-melanocyte-stimulating hormone (alpha-MSH), a potent anti-inflammatory cytokine in an ischaemic acute renal failure (ARF) rat model.
View Article and Find Full Text PDFMononuclear cells in subcutaneous haemorrhage with special consideration of myeloid percursor cells.
Med Sci Law
October 2000
Department of Legal Medicine, Medical University of Lübeck, Germany.
Various hematogenous markers were used to differentiate and quantify the types of mononuclear cells present in subcutaneous haemorrhages. Fifty samples of subcutaneous bleeding with a survival time of a few minutes to more than 48 hours were studied. The various cell types were detected using the following stains: Naphthol AS-D chloracetate esterase for myeloid cells, including mast cells; (alpha1-antichymotrypsin for monocytes/macrophages; UCHL1 for T-lymphocytes; and L26 for B lymphocytes.
View Article and Find Full Text PDFOrbital granulocytic sarcoma.
Br J Ophthalmol
December 1997
Department of Ophthalmology, McGill University, Montreal, Quebec, Canada.
Aim: Orbital granulocytic sarcoma is a localised tumour composed of cells of myeloid origin. Histological diagnosis can be difficult in patients with poorly differentiated orbital tumours and no evidence of systemic leukaemia. The naphthol AS-D chloracetate esterase (Leder stain) and immunohistochemical stains for lysozyme and MAC387 were used to determine the staining characteristics of these tumours.
View Article and Find Full Text PDF[Morphologic parameters for quantitative determination of inflammatory activity of the peritoneum].
Langenbecks Arch Chir
December 1997
Klinik für Chirurgie, Medizinische Universität zu Lübeck, Germany.
The morphology of the inflammatory activity of the peritoneum has been measured qualitatively but quantitative assessments are not common. In a standardized rat model we induced chronic abscess-forming peritonitis after laparotomy and inoculation of 2 ml Bacteroides fragilis suspension at a concentration of 10(9)/ml colony-forming units. The morphological inflammatory activity was determined quantitatively by staining the specimen of the peritoneum with naphthol-AS-D-chloracetate-esterase (NASDCE); through this staining the cytoplasm of granulocytes and tissue mast cells were marked.
View Article and Find Full Text PDFStudy on staining methods for mast cells in the nasal mucosa.
Auris Nasus Larynx
August 1992
Department of Otolaryngology, Asahikawa Medical School, Japan.
Mast cells are widely distributed in various organs. Two classes of mast cells, the mucosal mast cell (MMC) and the connective tissue mast cell (CTMC), have been shown to exist in the intestine of experimental animals. In the present study, we investigated the method of staining suitable for observing the mast cells distributing in the nasal mucosa, and also examined by the use of two fixatives whether the mast cells have properties of MMC or those of CTMC.
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