Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF01895294 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization by LC-MS/MS analysis of KLH vaccine conjugated with a tick antigen peptide.
Analyst
January 2025
Department of Proteomics, Mass Spectrometry Laboratory, Center for Genetic Engineering and Biotechnology, 31 Avenue, Cubanacan, Playa, Havana, Cuba.
Keyhole limpet haemocyanins (KLH1 and KLH2) from , are multi-subunit oxygen-carrying metalloproteins of approximately 3900 amino acids, that are widely used as carrier proteins in conjugate vaccines and in immunotherapy. KLHs and their derived conjugate vaccines are poorly characterized by LC-MS/MS due to their very stable supramolecular structures with megadalton molecular mass, and their resistance to efficient digestion with standard protocols. KLH1 and KLH2 proteins were conjugated to the conserved P0 peptide (pP0), derived from the P0 acidic ribosomal protein of sp.
View Article and Find Full Text PDFShrimp hemocyanin elicits a potent humoral response in mammals and is favorable to hapten conjugation.
Sci Rep
July 2024
Shanghai Epizyme BioMedical, Shanghai, China.
Conjugation to a carrier protein is essential to give rise to the antigenicity of hapten. Three carrier proteins e.g.
View Article and Find Full Text PDFCell Surface Vimentin Detection in Cancer Cells by Peptide-Based Monoclonal Antibody.
Avicenna J Med Biotechnol
January 2023
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Background: Vimentin is a prominent Intermediate Filaments (IFs) protein expressed in different mesenchymal origin cell types. Besides a wide range of cellular function roles associated with vimentin expression, its dysregulation and cell surface expression in the induction of malignancy properties have been reported extensively, making it a promising cancer-specific target. Therefore, this study aimed to generate and characterize anti-vimentin monoclonal antibodies.
View Article and Find Full Text PDFPurification and characterization of a hemocyanin with lectin-like activity isolated from the hemolymph of speckled shrimp, Metapenaeusmonoceros.
Biochimie
March 2023
Department of Biochemistry and Molecular Biology, School of Biological Sciences, Central University of Kerala, Tejaswini Hills, Periye, Kasargod, 671316, Kerala, India. Electronic address:
Lectins or agglutinins are mainly proteins or glycoproteins, reported to uphold an ability to agglutinate the red blood cells (RBCs) with a known sugar specificity in a diverse group of organisms. In the present study, we purified a hemocyanin (named as MmHc) from a shrimp, Metapenaeus monoceros by size-exclusion chromatography. Further characterization revealed that the purified MmHc showed hemagglutination activity that was found to be specifically inhibited by Lewis B and Lewis Y tetrasaccharides.
View Article and Find Full Text PDFChemically Modified Bacterial Sacculi as a Vaccine Microparticle Scaffold.
ACS Chem Biol
May 2022
Stanford ChEM-H, Stanford University, Stanford, California 94305, United States.
Vaccine scaffolds and carrier proteins increase the immunogenicity of subunit vaccines. Here, we developed, characterized, and demonstrated the efficacy of a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi. The PGN microparticles contain bio-orthogonal chemical handles allowing for site-specific attachment of immunogens.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!