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Human chitinolytic enzymes trigger growing interest, not only because a wide range of diseases and allergic responses are linked to chitinous components of pathogens, including their interplay with human enzymes, but also due to the increasing use of chitosans in biomedical applications. Here, we present a detailed side-by-side analysis of the only two human chitinases, chitotriosidase and acidic mammalian chitinase, as well as human lysozyme. By analyzing the cleavage of well-characterized chitosan polymers and defined chitin and chitosan oligomers, we report mild processivity and a quantitative subsite preference typical for GH18 chitinases for chitotriosidase and acidic mammalian chitinase.

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This study describes the applicability of the fluorescence polarization assay (FPA) based on the use of FITC-labeled oligosaccharide tracers of defined structure for the measurement of active lysozyme in hen egg white. Depending on the oligosaccharide chain length of the tracer, this method detects both the formation of the enzyme-to-tracer complex (because of lectin-like, i.e.

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Vitamin E nanoparticles enhance performance and immune status of Nile tilapia.

BMC Vet Res

December 2024

Fish Diseases Department, Animal Health Research Institute AHRI, Agriculture Research Centre ARC, Kafrelsheikh, Egypt.

Article Synopsis
  • This study investigates the effectiveness of nano-chitosan protected Vitamin E (VENPs) in enhancing the growth and immune response of Nile tilapia compared to regular Vitamin E and control groups.
  • Results showed that fish fed with VENPs had significantly better growth rates, lower feed conversion ratios, and improved immune responses, particularly at 75% and 100% concentrations.
  • Fish on the VENPs diet displayed a markedly lower mortality rate when challenged with A. hydrophila, suggesting that incorporating VENPs into their diet at 50, 75, and 100 mg/kg could boost their immunity against infections.
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Recent trends in the separation and analysis of chitooligomers.

Carbohydr Res

February 2025

Laboratory of Biotransformation, Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, CZ-14200, Prague, Czech Republic.

Chitosan is a widely used linear biopolymer composed mainly of glucosamine and to a lesser extent of N-acetylglucosamine units. Many biological activities of chitosan are attributed to its shorter oligomeric chains, which consist of chitosan prepared either by enzyme activity (lysozyme, bacterial chitinase) or chemically by acid-catalyzed hydrolysis (e.g.

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Remineralization and bacterial inhibition of early enamel caries surfaces by carboxymethyl chitosan lysozyme nanogels loaded with antibacterial drugs.

J Dent

January 2025

Tianjin Medical University School and Hospital of Stomatology & Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, No.12 Qixiangtai Road, Heping District, Tianjin 300070, PR China; Tianjin Medical University Institute of Stomatology, No.12 Qixiangtai Road, Heping District, Tianjin 300070, PR China. Electronic address:

Objective: This study aimed to develop dual-functional CMC-LYZ-ACP nanogels. Three different antibacterial substances, tea polyphenols (TPs), silver nitrate (AgNO), and chlorhexidine (CHX) are then combined to form three dual-functional CMC-LYZ-ACP nanogels for remineralization and antibacterial purposes.

Methods: An in vitro model of Streptococcus mutans biofilm was established to analyze the inhibitory effect of the antibacterial-remineralizing dual-functional nanogels on the biofilm.

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