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http://dx.doi.org/10.1016/0304-4165(66)90339-4 | DOI Listing |
Int J Biol Macromol
January 2025
Institute for Biology and Biotechnology of Plants, University of Münster, Schlossplatz 8, 48143 Münster, Germany. Electronic address:
Human chitinolytic enzymes trigger growing interest, not only because a wide range of diseases and allergic responses are linked to chitinous components of pathogens, including their interplay with human enzymes, but also due to the increasing use of chitosans in biomedical applications. Here, we present a detailed side-by-side analysis of the only two human chitinases, chitotriosidase and acidic mammalian chitinase, as well as human lysozyme. By analyzing the cleavage of well-characterized chitosan polymers and defined chitin and chitosan oligomers, we report mild processivity and a quantitative subsite preference typical for GH18 chitinases for chitotriosidase and acidic mammalian chitinase.
View Article and Find Full Text PDFBiomolecules
December 2024
Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia.
This study describes the applicability of the fluorescence polarization assay (FPA) based on the use of FITC-labeled oligosaccharide tracers of defined structure for the measurement of active lysozyme in hen egg white. Depending on the oligosaccharide chain length of the tracer, this method detects both the formation of the enzyme-to-tracer complex (because of lectin-like, i.e.
View Article and Find Full Text PDFBMC Vet Res
December 2024
Fish Diseases Department, Animal Health Research Institute AHRI, Agriculture Research Centre ARC, Kafrelsheikh, Egypt.
Carbohydr Res
February 2025
Laboratory of Biotransformation, Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, CZ-14200, Prague, Czech Republic.
Chitosan is a widely used linear biopolymer composed mainly of glucosamine and to a lesser extent of N-acetylglucosamine units. Many biological activities of chitosan are attributed to its shorter oligomeric chains, which consist of chitosan prepared either by enzyme activity (lysozyme, bacterial chitinase) or chemically by acid-catalyzed hydrolysis (e.g.
View Article and Find Full Text PDFJ Dent
January 2025
Tianjin Medical University School and Hospital of Stomatology & Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, No.12 Qixiangtai Road, Heping District, Tianjin 300070, PR China; Tianjin Medical University Institute of Stomatology, No.12 Qixiangtai Road, Heping District, Tianjin 300070, PR China. Electronic address:
Objective: This study aimed to develop dual-functional CMC-LYZ-ACP nanogels. Three different antibacterial substances, tea polyphenols (TPs), silver nitrate (AgNO), and chlorhexidine (CHX) are then combined to form three dual-functional CMC-LYZ-ACP nanogels for remineralization and antibacterial purposes.
Methods: An in vitro model of Streptococcus mutans biofilm was established to analyze the inhibitory effect of the antibacterial-remineralizing dual-functional nanogels on the biofilm.
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