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Analysis of Salicylic and Phenolic Acids in the Plant by HPLC-Fluorescence Detector.
Methods Mol Biol
January 2025
Natural Products Laboratory, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.
Salicylic acid is a member of benzoic acid derivatives, a group of compounds which have a backbone of C6C1 consisting of one carboxyl group and one (or more) hydroxyl group(s) attached to the aromatic ring. Salicylic acid is a signaling compound in systemic acquired resistance (SAR). An increased level of salicylic acid is found in the plant after a fungi's attack, which further induces the accumulation of phytoalexins, low molecular weight defense compounds.
View Article and Find Full Text PDFExtraction of Essential Oils and Terpene Volatiles from Plants and Identification by GC-MS-Based Techniques.
Methods Mol Biol
January 2025
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
The extraction of plant essential oils (EOs) and analysis by gas chromatography coupled to mass spectrometry (GC-MS) are standard methods when studying aromatic plants and the chemical composition of EOs. Here, two simple methods for the extraction of EO compounds from leaves of Thymus vulgaris are described. Organic solvent extraction and solid-phase microextraction (SPME), respectively, are used and the results of the GC-MS analyses are compared.
View Article and Find Full Text PDFBranched-chain and Aromatic Amino Acids in Blood and Seminal Plasma are Associated with Sperm Parameters; A Practice within a Fertility Clinic Considering the Metabolic Syndrome.
Reprod Sci
January 2025
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
The metabolomic approach has recently been used in the assessment of semen quality and male fertility. Additionally, the crucial roles of branched-chain amino acids (BCAAs) and aromatic amino acids (AAAs) in metabolic syndrome (MetS) were reported. However, little information exists about the association between BCAAs and AAAs with semen parameters, particularly in men with and without MetS.
View Article and Find Full Text PDFCryo-EM structures reveal the H/citrate symport mechanism of INDY.
Life Sci Alliance
April 2025
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea
I'm Not Dead Yet (INDY) functions as a transporter for citrate, a key metabolite in the citric acid cycle, across the plasma membrane. Partial deficiency of INDY extends lifespan, akin to the effects of caloric restriction. In this work, we use cryo-electron microscopy to determine structures of INDY in the presence and absence of citrate and in complex with the well-known inhibitor 4,4'-diisothiocyano-2,2'-disulfonic acid stilbene (DIDS) at resolutions ranging from 2.
View Article and Find Full Text PDFFish gut symbiotic bacterium Bacillus thuringiensis: RSM optimization for its extracellular lipase enzyme production, lipase-protein purification, characterization, and docking analysis.
Int J Biol Macromol
January 2025
State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu University of Technology, Chengdu 610059, Sichuan, China.
Lipase enzymes play a vital role in digestion and nutrient metabolism in host organisms, with symbiotic bacteria producing abundant enzymes, carbohydrates, vitamins, and other nutrients. This study aimed to isolate, identify, and screen lipase-producing bacteria from the gut of Systomus sarana, optimize enzyme production using Response Surface Methodology (RSM), and characterize the extracted lipase protein. A total of 11 bacterial strains were isolated and identified through 16S rRNA sequencing.
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