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Anal Chem
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Laboratory for Air Pollution/Environmental Technology, Empa, 8600 Dübendorf, Switzerland.
Mid-infrared laser absorption spectroscopy enables rapid and nondestructive analysis of methane clumped isotopes. However, current analytical methods require a sample size of 20 mL STP (0.82 mmol) of pure CH gas, which significantly limits its application to natural samples.
View Article and Find Full Text PDFA Potential Indicator Gene, tetM, to Assess Contamination by Antibiotic Resistance Genes in Greenhouses in South Korea.
Microbes Environ
January 2025
Center for Health Effects of Environmental Contamination, University of Iowa.
Antibiotic resistance genes (ARGs) have been emerging as a concerning threat to both environment and public health. The continuous input of manure, irrigation water, and fertilizers increases the abundance of ARGs in agricultural environments. However, current risk assessments have focused on clinical settings, which are not applicable to environmental settings.
View Article and Find Full Text PDFSequential STING and CD40 agonism drives massive expansion of tumor-specific T cells in liposomal peptide vaccines.
Cell Mol Immunol
January 2025
Department of Gastroenterology, Hepatology, Infectious Diseases and Endocrinology, Hannover Medical School, Hannover, Germany.
The clinical use of cancer vaccines is hampered by the low magnitude of induced T-cell responses and the need for repetitive antigen stimulation. Here, we demonstrate that liposomal formulations with incorporated STING agonists are optimally suited to deliver peptide antigens to dendritic cells in vivo and to activate dendritic cells in secondary lymphoid organs. One week after liposomal priming, systemic administration of peptides and a costimulatory agonistic CD40 antibody enables ultrarapid expansion of T cells, resulting in massive expansion of tumor-specific T cells in the peripheral blood two weeks after priming.
View Article and Find Full Text PDFThird generation sequencing transforming plant genome research: Current trends and challenges.
Gene
December 2024
Department of Molecular Biology and Biotechnology, Cotton University, Panbazar, Guwahati, Assam, 781001, India. Electronic address:
In recent years, third-generation sequencing (TGS) technologies have transformed genomics and transcriptomics research, providing novel opportunities for significant discoveries. The long-read sequencing platforms, with their unique advantages over next-generation sequencing (NGS), including a definitive protocol, reduced operational time, and real-time sequencing, possess the potential to transform plant genomics. TGS optimizes and enhances the efficiency of data analysis by removing the necessity for time-consuming assembly tools.
View Article and Find Full Text PDFChallenges in Describing Tremor and Dystonia.
Neurology
January 2025
Departments of Neurology, Human Genetics and Pediatrics, Emory University, Atlanta, GA.
Tremor is defined as an oscillatory and rhythmical movement. By contrast, dystonia is defined by sustained or intermittent abnormal postures, repetitive movements, or both. Tremor and dystonia often coexist in the same individual.
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