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Isolation of intact aortic valve scaffolds for heart-valve bioprostheses: extracellular matrix structure, prevention from calcification, and cell repopulation features.
J Biomed Mater Res A
December 2003
Department of Experimental Biomedical Sciences, University of Padova, Viale G, Colombo 3, I - 35121 Padova, Italy.
Extracellular matrix (ECM) scaffolds isolated from valvulated conduits can be useful in developing durable bioprostheses by tissue engineering provided that anatomical shape, architecture, and mechanical properties are preserved. As evidenced by SEM, intact scaffolds were derived from porcine aortic valves by the combined use of Triton X-100 and cholate (TRI-COL) or N-cetylpyridinium (CPC) and subsequent nucleic acid removal by nuclease. Both treatments were effective in removing most cells and all the cytomembranes, with preservation of (1) endothelium basal membranes, (2) ECM texture, including the D-periodical interaction of small proteoglycans with normally D-banded collagen fibrils, and (3) mechanical properties of the treated valves.
View Article and Find Full Text PDF[Percutaneous closure of congenital and iatrogenic coronary arterial fistulas].
G Ital Cardiol
November 1995
Laboratorio di Emodinamica Interventistica, Istituto di Cardiologia, Udine.
Coronary arteriovenous fistulas (CAF) are the most common hemodinamically significant congenital coronary anomalies. Surgery has been the only therapeutic option for ages. We describe three cases of percutaneous occlusion of CAF, congenital and iatrogenic, that we treated with different devices, to fit their different anatomic and functional characteristics.
View Article and Find Full Text PDF[Surgical treatment of deep venous reflux. Techniques, indications and results].
J Mal Vasc
January 1995
Service de Chirurgie et Médecine Vasculaire, Hôpital Rosthschild, Paris.
Introduction: complications of deep venous insufficiency can lead to surgery if one fails with medical treatment. The etiology can be primitive or secondary, the symptoms are identical. They go from heaviness of the legs to chronic leg ulcers.
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