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Sero-prevalence of anti-Leptospira antibodies and associated risk factors in rural Rwanda: A cross-sectional study.
PLoS Negl Trop Dis
December 2021
College of Medicine and Health Sciences, University of Rwanda, Kigali, Rwanda.
Background: Leptospirosis is a zoonotic disease transmitted through the urine of wild and domestic animals, and is responsible for over 50,000 deaths each year. In East Africa, prevalence varies greatly, from as low as 7% in Kenya to 37% in Somalia. Transmission epidemiology also varies around the world, with research in Nicaragua showing that rodents are the most clinically important, while studies in Egypt and Chile suggest that dogs may play a more important role.
View Article and Find Full Text PDFLeptospira spp in Rodents from Peridomestic Sites in Endemic Regions of Nicaragua.
Ecohealth
December 2020
Centro Veterinario de Diagnóstico e Investigación (CEVEDI), Escuela de Ciencias Agrarias y Veterinarias, Universidad Nacional Autónoma de Nicaragua-León, UNAN-León), Edificio Central, Contiguo a la Iglesia La Merced, Apartado Postal 68, León, Nicaragua.
Leptospirosis is one of the most important zoonoses in tropical countries, including Nicaragua, where it is considered endemic. The aim of this study was to determine the frequency of Leptospira spp in rodents captured from peridomestic sites in leptospirosis endemic regions of Nicaragua. Using live traps, 191 rodents were captured in 2012 and 2013 between April and December.
View Article and Find Full Text PDFEvaluation of three qPCR for the detection of pathogenic leptospires in domestic animals in Nicaragua.
Biomedica
December 2020
Centro Veterinario de Diagnóstico e Investigación, Escuela de Ciencias Agrarias y Veterinarias, Universidad Nacional Autónoma de Nicaragua, León, Nicaragua.
Introduction: Molecular biology diagnostic methods such as real-time PCR should be used in Nicaragua to improve the diagnosis of leptospirosis in humans and animals. Objective: To evaluate three qPCR methods for pathogenic Leptospira detection in domestic animals. Materials and methods: Real-time PCR primers were designed for the amplification of specific regions from the Lip 32 gene of Leptospira in SYBER Green (SYBER Green-A) and TaqMan, as well in SYBER Green-B as previously published.
View Article and Find Full Text PDFDetection of Pathogenic Leptospires in Water and Soil in Areas Endemic to Leptospirosis in Nicaragua.
Trop Med Infect Dis
September 2020
Centro Veterinario de Diagnóstico e Investigación (CEVEDI), Departamento de Veterinaria y Zootecnia, Escuela de Ciencias Agrarias y Veterinarias, Universidad Nacional Autónoma de Nicaragua-León (UNAN-León), Carretera a La Ceiba 1 Km al Este, León 21000, Nicaragua.
In Nicaragua, there are ideal environmental conditions for leptospirosis. The objective of this investigation was to detect pathogenic and saprophytic leptospires in water and soil samples from leptospirosis-endemic areas in Nicaragua. Seventy-eight water and 42 soil samples were collected from houses and rivers close to confirmed human cases.
View Article and Find Full Text PDFInvestigating Possible Infectious Causes of Chronic Kidney Disease of Unknown Etiology in a Nicaraguan Mining Community.
Am J Trop Med Hyg
September 2019
Department of Epidemiology, Boston University School of Public Health, Boston, Massachusetts.
A chronic kidney disease of unknown etiology (CKDu) has been killing workers in Central America. Occupational heat stress is thought to play an important role. Leptospirosis and hantavirus have been suggested as additional possible risk factors.
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