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Nonemissive Iridium(III) Solvent Complex as a Self-Reporting Photosensitizer for Monitoring Phototherapeutic Efficacy in a "Signal on" Mode.
Chem Biomed Imaging
December 2024
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, P. R. China.
Photodynamic therapy (PDT) has long been receiving increasing attention for the minimally invasive treatment of cancer. The performance of PDT depends on the photophysical and biological properties of photosensitizers (PSs). The always-on fluorescence signal of conventional PSs makes it difficult to real-time monitor phototherapeutic efficacy in the PDT process.
View Article and Find Full Text PDFCloning, purification and characterization of a novel thermostable recombinant tannase from Galactobacillus timonensis.
Enzyme Microb Technol
December 2024
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang 330047, China.
The exorbitant production costs associated with natural tannases pose a significant challenge to their widespread industrial utilization. Microbial expression systems provide a cost-effective method for enzyme production. In this study, a putative gene encoding the subtype B tannase (Gt-Tan) was cloned from Galactobacillus timonensis and expressed heterologously in Escherichia coli BL21 (DE3) cells.
View Article and Find Full Text PDFA green method on dipole solvent as "Activators": γ-valerolactone/HO system promoted degradation of ciprofloxacin by ferrate(Ⅵ).
Water Res
December 2024
Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China.
This paper investigates the efficient degradation of ciprofloxacin (CIP) in a sustainable γ-valerolactone (GVL) and water (H₂O) mixed system by controlling proton transfer and reducing the self-decay rate of Fe(VI). The kinetic model reveals that the GVL/H₂O system exhibits a rate constant of (9.7 ± 0.
View Article and Find Full Text PDFExpression Variations of Chloride Ion Channel Related Genes During Diapause Termination in Silkworm, Bombyx mori.
Arch Insect Biochem Physiol
December 2024
Department of Sericultural Science, College of Animal Science, South China Agricultural University, Guangzhou, China.
Instant and refrigerated acid soaking are commonly used in cocoon production to prevent or break diapause, and provide developable silkworm eggs for sericulture, while their mechanisms have not been fully understood. This study aims to investigate the mechanisms by which hydrochloric acid (HCl) or dimethyl sulfoxide (DMSO) promotes embryonic development in silkworm Bombyx mori, focusing on the chloride ion (Cl) related gene expression profiles. Our results revealed that the HCl treatment of up to 6 min enhanced hatchability in freshly picked and cold-stored eggs, whereas a slight decrease in hatchability was observed in those treated with DMSO for 40 min.
View Article and Find Full Text PDFNa-Complexed Dendritic Polyglycerols for Recovery of Frozen Cells and Their Network in Media.
Adv Mater
December 2024
Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, Republic of Korea.
In this study, a novel phenomenon is identified where precise control of topology and generation of polyglycerol induce the retention of Na ions in biological buffer systems, effectively inhibiting ice crystal growth during cryopreservation. Unlike linear and hyperbranched counterparts, densely-packed hydroxyl and ether groups in 4th-generation dendritic polyglycerol interact with the ions, activating the formation of hydrogen bonding at the ice interface. By inhibiting both intra- and extracellular ice growth and recrystallization, this biocompatible dendritic polyglycerol proves highly effective as a cryoprotectant; hence, achieving the cell recovery rates of ≈134-147%, relative to those of 10% dimethyl sulfoxide, which is a conventional cryoprotectant for human tongue squamous carcinoma (HSC-3) cell line and human umbilical vein endothelial (HUVEC) cells.
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