Camel myoglobin.

Biochem J

Published: March 1966

1. Crystalline myoglobin was prepared from camel heart muscle. 2. A method was developed for the isolation of myoglobin that employs molecular-sieve chromatography. 3. Analytical chromatography of the camel myoglobin on a molecular-sieve column and on two types of ion-exchange columns gave in each case a single elution band, which accounted for better than 98% recovery and showed that the product was free from haemoglobin. 4. The iron content on a dry weight basis was 0.308%. This value corresponds to a molecular weight of 18100. 5. The spectra of acidic ferrimyoglobin, basic ferrimyoglobin and ferrimyoglobin cyanide were measured. 6. The pK(a) of the dissociation of the haem-bound water molecule in acidic ferrimyoglobin was 8.53 at 25 degrees . 7. Conclusions are drawn about the charge on the surface of the camel ferrimyoglobin molecule as compared with horse and sperm-whale ferrimyoglobins.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1264936PMC
http://dx.doi.org/10.1042/bj0980909DOI Listing

Publication Analysis

Top Keywords

camel myoglobin
8
acidic ferrimyoglobin
8
ferrimyoglobin
5
camel
4
myoglobin crystalline
4
crystalline myoglobin
4
myoglobin prepared
4
prepared camel
4
camel heart
4
heart muscle
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!