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Extracellular phospholipase, protease and hemolysin production by strains of opportunistic yeasts from the excreta of Mew Gulls breeding in natural and urban habitats.
Vet Res Commun
January 2025
Soil Science Faculty, Lomonosov Moscow State University, Moscow, 119234, Russia.
Extracellular hydrolytic activity (phospholipase, protease and hemolysin production) was evaluated in 178 strains of potentially pathogenic ascomycetous (Candida parapsilosis, Candida tropicalis) and basidiomycetous (Rhodotorula mucilaginosa) yeasts isolated from the excreta of Mew Gulls. Two bird colonies, one nesting in a natural habitat and the other in an urban habitat at the landfill, were studied simultaneously during their 7-month breeding season. Significant differences in phospholipase and protease production were found between natural and anthropophized strains.
View Article and Find Full Text PDFRecombinant Fungal Aspartic Endopeptidases: Insights into Protein Hydrolysis and Combined Effect with Pepsin for Animal Feed Application.
J Agric Food Chem
January 2025
Faculty of Mechanical and Process Engineering, Hochschule Offenburg, 77652 Offenburg, Germany.
Protein hydrolysis under acidic conditions can improve the product quality, nutrient availability, and cost efficiency, particularly when neutral or alkaline enzymes are ineffective. Six fungal aspartic endopeptidases (FAPs) were recombinantly expressed as active enzymes in , with peak activity between 30-50 °C and pH 3.0-4.
View Article and Find Full Text PDFLipidomics and Flavouromics assessment of the effects of enzyme modification on butter composition.
Food Chem
December 2024
College of Food Science and Engineering, Wuhan Polytechnic University, Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan 430048, China.. Electronic address:
Enzyme-modified butter (EMB) is well-known for its rich flavour, which is primarily defined by the process of enzymatic hydrolysis. This study employed lipidomics and flavouromics to assess the differences between EMBs and to uncover the intrinsic links between volatile organic compounds (VOCs) and lipids. Approximately 273 lipids and 82 VOCs were identified in butter.
View Article and Find Full Text PDFNovel methyldithiocarbazate derivatives as NDM-1 inhibitors to combat multidrug-resistant bacterial infection with β-lactams.
Bioorg Chem
December 2024
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun 130062, China. Electronic address:
Given the ever-evolving landscape of antimicrobial resistance, the emergence of New Delhi metallo-β-lactamase-1 (NDM-1) has introduced a formidable challenge to global public health. In previous research, we identified the Compound Zndm19 as an NDM-1 inhibitor and reported Zndm19 derivatives, which exhibited moderate antibacterial activity when combined with meropenem (MEM). This moderate activity may have been due to the inability of Zndm19 to efficiently penetrate the bacterial outer membrane or its susceptibility to hydrolysis, which prevented it from exerting strong enzyme inhibition in synergy with bacterial cells.
View Article and Find Full Text PDFAssessing the impact of arsenic on symbiotic and free-living PGPB: plant growth promoting traits, bacterial compatibility and adhesion on soybean seed.
World J Microbiol Biotechnol
December 2024
Departamento de Biología Molecular, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto (UNRC), Ruta Nacional 36 Km 601, 5800, Río Cuarto, Córdoba, CP, Argentina.
Arsenic (As) contamination in agricultural groundwater and soil is a significant economic and health problem worldwide. It inhibits soybean (Glycine max (L.) Merr.
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