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Acoustic resonance effects and cavitation in SAW aerosol generation.
Ultrason Sonochem
August 2023
SAWLab Saxony, Leibniz Institute for Solid State and Materials Research Dresden, Helmholtzstr. 20, Dresden 01069, Germany.
The interaction of surface acoustic waves (SAWs) with liquids enables the production of aerosols with adjustable droplet sizes in the micrometer range expelled from a very compact source. Understanding the nonlinear acousto-hydrodynamics of SAWs with a regulated micro-scale liquid film is essential for acousto-microfluidics platforms, particularly aerosol generators. In this study, we demonstrate the presence of micro-cavitation in a MHz-frequency SAW aerosol generation platform, which is touted as a leap in aerosol technology with versatile application fields including biomolecule inhalation therapy, micro-chromatography and spectroscopy, olfactory displays, and material deposition.
View Article and Find Full Text PDFNi(II) functionalized polyhedral oligomeric silsesquioxane based capillary monolith for purification of histidine-tagged proteins by immobilized metal affinity micro-chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci
June 2023
Hacettepe University, Graduate School of Science and Engineering, Division of Bioengineering, Ankara, Turkey; Hacettepe University, Chemical Engineering Department, Ankara, Turkey. Electronic address:
A new capillary monolithic stationary phase was synthesized for the purification of histidine tagged proteins by immobilized metal affinity micro-chromatography (μ-IMAC). For this purpose, mercaptosuccinic acid (MSA) linked-polyhedral oligomeric silsesquioxane [MSA@poly(POSS-MA)] monolith 300 μm in diameter was obtained by thiol-methacrylate polymerization using methacryl substituted-polyhedral oligomeric silsesquioxane (POSS-MA) and MSA as the thiol functionalized agent in a fused silica capillary tubing. Ni(II) cations were immobilized onto the porous monolith via metal-chelate complex formation with double carboxyl functionality of bound MSA segments.
View Article and Find Full Text PDFIncorporation of automated buffer exchange empowers high-throughput protein and plasmid purification for downstream uses.
SLAS Technol
August 2023
Integrated Micro-Chromatography Systems, Inc., 110 Centrum Drive, Irmo, SC 29063, United States. Electronic address:
The continued acceleration of time-to-market product development and rising demand for biotherapeutics have hastened the need for higher throughput within the biopharmaceutical industry. Automated liquid handlers (ALH) are increasingly popular due to flexible programming that enables processing of multiple samples with an array of functions. This flexibility is useful in streamlining research that requires chromatographic procedures to achieve product purity for downstream analysis.
View Article and Find Full Text PDFInsight into the Ozone-Assisted Low-Temperature Combustion of Dimethyl Ether by Means of Stabilized Cool Flames.
J Phys Chem A
October 2021
University of Lille, CNRS, UMR 8522-PC2A-Physicochimie des Processus de Combustion et de l'Atmosphère, F-59000 Lille, France.
The low-temperature combustion kinetics of dimethyl ether (DME) were studied by means of stabilized cool flames in a heated stagnation plate burner configuration using ozone-seeded premixed flows of DME/O. Direct imaging of CHO* chemiluminescence and laser-induced fluorescence of CHO were used to determine the flame front positions in a wide range of lean and ultra-lean equivalence ratios and ozone concentrations for two strain rates. The temperature and species mole fraction profiles along the flame were measured by coupling thermocouples, gas chromatography, micro-chromatography, and quadrupole mass spectrometry analysis.
View Article and Find Full Text PDFIntegrating Plasmonic Supercrystals in Microfluidics for Ultrasensitive, Label-Free, and Selective Surface-Enhanced Raman Spectroscopy Detection.
ACS Appl Mater Interfaces
October 2020
CINBIO, Universidade de Vigo, Campus Universitario Lagoas, Marcosende, 36310 Vigo, Spain.
Surface-enhanced Raman spectroscopy (SERS) microfluidic chips for label-free and ultrasensitive detection are fabricated by integrating a plasmonic supercrystal within microfluidic channels. This plasmonic platform allows the uniform infiltration of the analytes within the supercrystal, reaching the so-called hot spots. Moreover, state-of-the-art simulations performed using large-scale supercrystal models demonstrate that the excellent SERS response is due to the hierarchical nanoparticle organization, the interparticle separation (IPS), and the presence of supercrystal defects.
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