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Protocol for muscle fiber type and cross-sectional area analysis in cryosections of whole lower mouse hindlimbs.
STAR Protoc
December 2024
Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Capital Region, Denmark. Electronic address:
We outline a protocol to visualize all mouse lower hindlimb skeletal muscles simultaneously. We describe procedures for orientating the whole lower hindlimb in gum tragacanth prior to freezing, simplifying the proceeding experimental steps, and enhancing the comprehensiveness of characterizations. We then detail steps for quantifying muscle fiber size and fiber type characteristics in a single cryosection using histochemistry and immunofluorescence.
View Article and Find Full Text PDFProtocol for muscle fiber type and cross-sectional area analysis in cryosections of whole lower mouse hindlimbs.
bioRxiv
August 2024
Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen.
We outline a robust, simple, and cost-effective method to simultaneously visualize all mouse lower hindlimb skeletal muscles. We describe procedures for orientating the whole lower hindlimb in gum tragacanth prior to freezing, simplifying the proceeding experimental steps, and enhancing the clarity and comprehensiveness of characterizations. We then detail steps for quantifying muscle fiber size and fiber type characteristics in a single cryosection using immunofluorescence and histochemistry.
View Article and Find Full Text PDFIntraosseous mandibular clear cell odontogenic carcinoma with predominant small round blue cells: a potential diagnostic pitfall.
Oral Surg Oral Med Oral Pathol Oral Radiol
December 2024
Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Objective: Clear cell odontogenic carcinoma (CCOC) is a rare malignancy of the jaw, presenting significant diagnostic challenges. This report aims to highlight the complexities associated with biopsy-based diagnoses of oral and maxillofacial lesions, as demonstrated in a case of intraosseous mandibular CCOC initially suggestive of Ewing's sarcoma due to its presentation with small round blue cells.
Results: The patient, a 37-year-old male, presented with a mandibular lesion that on incisional biopsy was suggestive of Ewing's sarcoma.
Perivascular epithelioid cell tumor (PEComa) is a mesenchymal tumor thought to originate from perivascular epithelioid cells (PECs). The normal counterpart to PEC, however, has not been identified in any human organ, and the debate as to whether PEComa is related to smooth muscle tumors has persisted for many years. The current series characterizes 4 cases of uterine leiomyosarcoma (LMS) coexisting with PEComas.
View Article and Find Full Text PDFGlycogenic acanthosis of the tongue.
Indian J Pathol Microbiol
July 2024
Department of Oral Pathology and Microbiology, Subharti Dental College and Hospital, Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India.
Glycogenic acanthosis (GA) is a benign lesion characterized by elevated, small, white plaques commonly detected in the esophageal mucosa. To date, only 6 cases have been reported in the English literature documenting its involvement in the oral cavity. Herein we report a case of large GA on the ventrolateral aspect of the tongue in a 42-year-old male (third case reported on the tongue).
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