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Cellular milk production: Proteins and minerals in secretomes from cultivated bovine milk-derived mammary cells.
Food Chem
March 2025
Department of Food Science, Aarhus University, Agro Food Park 48, DK-8200 Aarhus N, Denmark.
This study explores the feasibility of utilizing in vitro cultivated milk-derived bovine mammary epithelial cells (bMECs) for the production of milk constituents. BMECs were isolated from milk and treated with various lactogenic agents in 3D transwell systems. By proteomics, >900 proteins were identified and quantified in the secretomes, including >100 milk-related proteins such as caseins and enzymes.
View Article and Find Full Text PDFLoss of STING impairs lactogenic differentiation.
Development
October 2024
Department of Veterinary Physiology & Pharmacology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA.
Heightened energetic and nutrient demand during lactogenic differentiation of the mammary gland elicits upregulation of various stress responses to support cellular homeostasis. Here, we identify the stimulator of interferon genes (STING) as an immune supporter of the functional development of mouse mammary epithelial cells (MECs). An in vitro model of MEC differentiation revealed that STING is activated in a cGAS-independent manner to produce both type I interferons and proinflammatory cytokines in response to the accumulation of mitochondrial reactive oxygen species.
View Article and Find Full Text PDFNuclear factor erythroid 2-related factor 1 regulates the expression of proteasomal genes in ketotic cows and protects mammary cells against free fatty acid-induced endoplasmic reticulum stress.
J Dairy Sci
January 2025
National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address:
Ketosis is a common metabolic disorder in high-yielding cows and is characterized by high concentrations of BHB and free fatty acids (FFA). High concentrations of FFA induce endoplasmic reticulum (ER) stress in multiple organs including mammary tissue, and result in reduced milk production and lower milk quality. In nonruminants, loss of nuclear factor erythroid 2-related factor 1 (NFE2L1) results in ER stress.
View Article and Find Full Text PDFDevelopment and characterisation of a novel inhibitory anti-GH monoclonal antibody.
J Mol Endocrinol
January 2024
Liggins Institute, University of Auckland, Auckland, New Zealand.
Excess growth hormone (GH) has been implicated in multiple cancer types and there is increasing interest in the development of therapeutic inhibitors targeting GH-GH receptor (GHR) signalling. Here we describe a panel of anti-GH monoclonal antibodies (mAbs) generated using a hybridoma approach and identify two novel inhibitory mAbs (1-8-2 and 1-46-3) that neutralised GH signalling. mAbs 1-8-2 and 1-46-3 exhibited strong inhibitory activity against GH-dependent cell growth in a Ba/F3-GHR cell viability assay, with EC50 values of 1.
View Article and Find Full Text PDFSuccessful editing and maintenance of lactogenic gene expression in primary bovine mammary epithelial cells.
In Vitro Cell Dev Biol Anim
May 2023
Applied Translational Genetics Group, School of Biological Sciences, University of Auckland, Auckland, New Zealand.
In vitro investigation of bovine lactation processes is limited by a lack of physiologically representative cell models. This deficiency is most evident through the minimal or absent expression of lactation-specific genes in cultured bovine mammary tissues. Primary bovine mammary epithelial cells (pbMECs) extracted from lactating mammary tissue and grown in culture initially express milk protein transcripts at relatively representative levels.
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