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Solution-phase nucleic acid reaction weaves interfacial barriers on unmodified electrodes: Just-in-time generation of sensor interface for convenient and highly sensitive bioassays.
Talanta
January 2025
The Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, China. Electronic address:
Electrochemical bioassays that rely on sensor interfaces based on immobilized DNA probes often encounter challenges such as complex fabrication processes and limited binding efficiency. In this study, we developed a novel electrochemical bioassay that bypasses the need for probe immobilization by employing a solution-phase nucleic acid reaction to create interfacial barriers on unmodified electrodes, enabling rapid, just-in-time sensor interface formation. Specifically, a 3'-phosphorylated recognition probe was used to identify the target microRNA-21 (miR-21), followed by target recycling facilitated by duplex-specific nuclease (DSN), which resulted in extensive hydrolysis of the recognition probe into DNA fragments with 3'-hydroxyl ends.
View Article and Find Full Text PDFSynthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
Free Radic Biol Med
December 2024
Laboratory of Reproductive Endocrinology, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-4-4, Kagamiyama, Higashihiroshima, Hiroshima, 7398528, Japan. Electronic address:
Sperm cells are highly susceptible to oxidative stress, which decreases their motility and fertility. However, glutathione (GSH) plays a critical role in protecting sperm cells from oxidative damage, a common byproduct of mitochondrial oxidative phosphorylation. On the other hand, GSH biosynthesis in sperm is limited by the availability of cysteine (Cys), which is inherently unstable and found at low concentrations in boar seminal plasma.
View Article and Find Full Text PDFThe Vsr-like protein FASTKD4 regulates the stability and polyadenylation of the MT-ND3 mRNA.
Nucleic Acids Res
December 2024
The Kids Research Institute Australia, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, Western Australia 6009, Australia.
Expression of the compact mitochondrial genome is regulated by nuclear encoded, mitochondrially localized RNA-binding proteins (RBPs). RBPs regulate the lifecycles of mitochondrial RNAs from transcription to degradation by mediating RNA processing, maturation, stability and translation. The Fas-activated serine/threonine kinase (FASTK) family of RBPs has been shown to regulate and fine-tune discrete aspects of mitochondrial gene expression.
View Article and Find Full Text PDFTranscriptomics, metabolomics and proteomics analyses reveal glyphosate tolerance mechanism in red swamp crayfish Procambarus clarkii.
Sci Total Environ
January 2025
Key Laboratory of Integrated Rice-Fish Farming Ecosystem, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Aquaculture Engineering and Technology Research Centre, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
Glyphosate (Gly), the world's most widely used herbicide in agriculture, can poison the red swamp crayfish, Procambarus clarkii, via spray drift and surface runoff into surface waters. However, there is a paucity of research on the mechanisms that affect crayfish tolerance to Gly at typical environmental concentrations. To address this research gap, we investigated the effects of Gly stress (0, 6, 12, 24, and 72 h) at different concentrations (0, 1.
View Article and Find Full Text PDFAn improved bacterial single-cell RNA-seq reveals biofilm heterogeneity.
Elife
December 2024
The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Medical Research Institute, Wuhan University, Wuhan, China.
In contrast to mammalian cells, bacterial cells lack mRNA polyadenylated tails, presenting a hurdle in isolating mRNA amidst the prevalent rRNA during single-cell RNA-seq. This study introduces a novel method, ribosomal RNA-derived cDNA depletion (RiboD), seamlessly integrated into the PETRI-seq technique, yielding RiboD-PETRI. This innovative approach offers a cost-effective, equipment-free, and high-throughput solution for bacterial single-cell RNA sequencing (scRNA-seq).
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