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Newly formed skeletal muscle fibers are prone to false positive immunostaining by rabbit antibodies.
Acta Histochem
January 2011
Department of Immunology and Microbiology, University of Southern Denmark, Odense C, Denmark.
Reports on muscle biology and regeneration often implicate immuno(cyto/histo)chemical protein characterization using rabbit polyclonal antibodies. In this study we demonstrate that newly formed myofibers are especially prone to false positive staining by rabbit antibodies and this unwanted staining is only recognized (1) by a negative muscle tissue control that does not harbor the protein to be examined (fx. from knockout mouse) or (2) by use of a nonsense rabbit antibody that has been prepared in the same way as the antibody of interest.
View Article and Find Full Text PDFImmunoelectrophoretic studies on pig intestinal brush border proteins.
Biochim Biophys Acta
October 1977
Brush borders were prepared from pig intestinal mucosa and the membrane proteins solubilized with either Triton X-100 or papain. Proteins, thus released, were used as antigens to raise antisera in rabbits. The immunoglobulin G fractions were isolated and shown by the double layer immunofluorescence staining technique to react only with the brush border region of the enterocyte.
View Article and Find Full Text PDFThere has been many studies on myocardial catecholamine (CA) in congestive heart failure and ishemias heart disease. However, it has been mainly studied pharmacologically and biochemically and has not been elucidated completely the local change of CA of the myocardium. CA in sympathetic nerves was first stained fluorescence histochemically by Falck-Hillarp in 1962, and many observations were made on its distribution and morphologically concentration of CA in tissue was also observed.
View Article and Find Full Text PDF[Histo-chemical preparation and microphotography of tissue cultures].
Zentralbl Bakteriol Orig
December 1964
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