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Comparative electron microscopic visualization of the lung alveolar epithelial glycocalyx with different staining and labeling methods.
J Anat
September 2024
Institute of Functional Anatomy, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Article Synopsis
- * The study aimed to improve visualization techniques for this glycocalyx using chemical fixation and various staining methods in mice, comparing agents like alcian blue, ruthenium red, and lanthanum nitrate.
- * Results showed that alcian blue provided the best staining, while the study's methods also align with animal welfare principles by promoting reduction and refinement in the use of animals for research.
Fibronectin binds integrin α5β1 to regulate macular neovascularization through the Wnt/β-catenin signaling pathway.
Exp Eye Res
May 2024
Department of Ophthalmology, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China. Electronic address:
Article Synopsis
- Age-related macular degeneration (AMD) is a leading cause of vision loss among older adults, primarily due to macular neovascularization (MNV) and subretinal fibrosis, which are linked to changes in extracellular matrix protein composition.
- The study investigates the role of fibronectin (FN) and its receptor, integrin α5β1, in promoting MNV, using mouse models and a cell line to analyze FN expression and its molecular mechanisms through various assays.
- Results showed increased FN and α5β1 levels in models of MNV, and silencing FN led to reduced cell migration and blood vessel formation, indicating FN's significant role in the development of AMD and its potential targeting for treatment.
[LPS-induced endothelial cytoskeleton remodeling in human lung vessels and related miRNAs-profiling].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
July 2023
Intensive Care Unit, Cardiocerebral Vascular Disease Hospital, General Hospital of Ningxia Medical University, Yinchuan 750012, China. *Corresponding author, E-mail: mxwei-99@ 163.com.
Objective To investigate the effects of lipopolysaccharide (LPS) on human pulmonary vascular endothelial cells (HPVECs) cytoskeleton and perform biological analysis of the microRNA (miRNA) spectrum. Methods The morphology of HPVECs was observed by microscope, the cytoskeleton by FITC-phalloidin staining, and the expression of VE-cadherin was detected by immunofluorescence cytochemical staining; the tube formation assay was conducted to examine the angiogenesis, along with cell migration test to detect the migration, and JC-1 mitochondrial membrane potential to detect the apoptosis. Illumina small-RNA sequencing was used to identify differentially expressed miRNAs in NC and LPS group.
View Article and Find Full Text PDFRetinoic acid induces the osteogenic differentiation of cat adipose tissue-derived stromal cells from distinct anatomical sites.
J Anat
February 2023
Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal.
Mesenchymal stromal cells-based regenerative orthopedic therapies have been used in cats as a promising and innovative therapeutic approach to enhance the repair of bone defects. Adipose tissue-derived stromal cells (ADSCs) can be obtained from two main sites-subcutaneous and visceral-with established differences regarding structure, composition, cell content, and functionality. However, in cats, to the best of the authors' knowledge, no studies have been conducted to compare the functional activity of the ADSCs isolated from the two sites, and the impact of these differences on the induced osteogenic potential.
View Article and Find Full Text PDFCaMKII is a modulator in neurodegenerative diseases and mediates the effect of androgen on synaptic protein PSD95.
Front Genet
August 2022
Department of Anatomy, Hebei Medical University, Shijiazhuang, China.
Androgens rapidly regulate synaptic plasticity in hippocampal neurones, but the underlying mechanisms remain unclear. In this study, we carried out a comprehensive bioinformatics analysis of functional similarities between androgen receptor (AR) and the synaptic protein postsynaptic density 95 (PSD95) to evaluate the effect. Using different measurements and thresholds, we obtained consistent results illustrating that the two proteins were significantly involved in similar pathways.
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