The deuterated phospholipid, 1,2-dipalmitoyl-d62-phosphatidylcholine is shown by Raman spectroscopic measurements to be useful for obtaining information concerning phospholipid conformation in complex phospholipid and lipidprotein mixtures. The Raman bands of the deuterated phospholipid are assigned, and the sensitivity of these vibrational modes to conformational changes in the bilayer is demonstrated. Deuteration of the alkyl chains reveals the CH vibrations of the head group. A change in these bands is observed at the melting temperature and is assigned to alteration of the glycerol backbone conformation upon melting.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1473445 | PMC |
| http://dx.doi.org/10.1016/S0006-3495(78)85484-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Elucidating the conformational behavior and membrane-destabilizing capability of the antimicrobial peptide ecPis-4s.
Biophys Chem
February 2025
Departamento de Química, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 39100-000 Diamantina, MG, Brazil. Electronic address:
Here we present studies of the structure and membrane interactions of ecPis-4 s, a new antimicrobial peptide from the piscidin family, which shows a wide-range of potential biotechnological applications. In order to understand the mode of action ecPis-4 s, the peptide was chemically synthesized and structural investigations in the presence of anionic POPC:POPG (3:1, mol:mol) membrane and SDS micelles were performed. CD spectroscopy demonstrated that ecPis-4 s has a high content of helical structure in both membrane mimetic media, which is in line with solution NMR spectroscopy that revealed an amphipathic helical conformation throughout the entire peptide chain.
View Article and Find Full Text PDFA simultaneous analysis strategy of glycerophospholipids and lipid mediators based on secondary extraction scheme.
Anal Bioanal Chem
January 2025
State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources, Ding11 Xueyuan Road, Beijing, 100083, China.
Lipid peroxidation plays an important role in physiological and pathophysiological conditions; it occurs mainly in the vicinity of the membrane. Glycerophospholipids are indispensable for normal structure of membranes. Lipid mediators are final products of lipid peroxidation.
View Article and Find Full Text PDFDecomposition of mixed DMPC-aescin vesicles to bicelles is linked to the lipid's main phase transition: A direct evidence by using chain-deuterated lipid.
J Colloid Interface Sci
February 2025
Physical and Biophysical Chemistry, Bielefeld University, Universitätstraße 25, Bielefeld, 33615, Germany. Electronic address:
This work investigates the conversion of bicelles into larger sheets or closed vesicles upon dilution and temperature increase for a system composed of the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and the saponin aescin. Due to its peculiar amphiphilic character, aescin is able to decompose DMPC bilayers into smaller, rim-stabilized bicelles. Aspects of the transition process are analyzed in an aescin content- and temperature-dependent manner by photon correlation spectroscopy (PCS), turbidimetry and small-angle neutron scattering (SANS).
View Article and Find Full Text PDFLipid Compositions of Liquid-Ordered and Liquid-Disordered Phases in Ternary Membranes of Sphingomyelin, Cholesterol, and Dioleoylphosphatidylcholine Determined by H NMR: Stearoyl-Sphingomyelin Compared with Its Palmitoyl Counterpart.
Langmuir
October 2024
Graduate School of Science, Osaka University, Machikaneyama 1-1, Toyonaka, Osaka 560-0043, Japan.
Sphingomyelin (SM) and cholesterol are the major lipids in the signaling platforms of cell membranes, known as lipid rafts. In particular, SM with a stearoyl chain (C18-SM) is abundant in specific tissues such as the brain, the most cholesterol-rich organ, whereas the distribution of palmitoyl (C16)-SM is ubiquitous. Here, we reveal the differences between palmitoyl- and stearoyl-SM in lipid-lipid interactions based on the tie lines obtained from the H solid-state NMR spectra of bilayer systems composed of SM/dioleoylphosphatidylcholine/cholesterol 33:33:33 and 40:40:20.
View Article and Find Full Text PDFProbing Deuteration-Induced Phase Separation in Supported Lipid Monolayers using Hyperspectral TERS Imaging.
J Phys Chem Lett
October 2024
Department of Chemistry and Applied Biosciences, ETH Zurich, 8093 Zurich, Switzerland.
In this study, we investigate the impact of deuteration on the formation of phase-separated domains in supported lipid monolayers using hyperspectral Tip-Enhanced Raman Spectroscopy (TERS) imaging. The intricate organization of biological membranes plays a crucial role in cellular functions. Various factors that influence domain formation have been identified in previous studies such as lipid tail length and cholesterol concentration.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!