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SARS-CoV-2 NSP3/4 control formation of replication organelle and recruitment of RNA polymerase NSP12.
J Cell Biol
March 2025
Guangzhou National Laboratory , Guangzhou, China.
β-coronavirus rearranges the host cellular membranes to form double-membrane vesicles (DMVs) via NSP3/4, which anchor replication-transcription complexes (RTCs), thereby constituting the replication organelles (ROs). However, the impact of specific domains within NSP3/4 on DMV formation and RO assembly remains largely unknown. By using cryogenic-correlated light and electron microscopy (cryo-CLEM), we discovered that the N-terminal and C-terminal domains (NTD and CTD) of SARS-CoV-2 NSP3 are essential for DMV formation.
View Article and Find Full Text PDFEnhancing Digestibility and Intestinal Peptide Release of Protein: An Enzymatic Approach.
J Fungi (Basel)
December 2024
College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China.
is a tasty and low-calorie mushroom containing abundant high-quality protein. This study aims to improve the digestibility of protein (PEP) and hence to facilitate its development as a healthy alternative protein. The extracted PEP was pretreated with 1000-5000 U of papain, neutral protease and alkaline protease.
View Article and Find Full Text PDFLyophilized powder of calf bone marrow hydrolysate liposomes improved renal anemia: In vitro and in vivo evaluation.
PLoS One
December 2024
Department of Research and Development, Jinan Perfect Biological Technology Co., LTD, Jinan, Shandong, China.
This study aimed to find whether oral administration of calf bone marrow hydrolysate liposomes (CBMHL) can improve renal anemia. Calf bone marrow was defatted, papain hydrolyzed, liposomalized and lyophilized. Its hematopoietic ability was proved by the colony formation experiment of umbilical cord blood hematopoietic stem cells in vitro.
View Article and Find Full Text PDFRegulation of N-degron recognin-mediated autophagy by the SARS-CoV-2 PLpro ubiquitin deconjugase.
Autophagy
January 2025
Department of Cell and Molecular Biology, Karolinska Institutet, Solna, Sweden.
Viral proteases play critical roles in the host cell and immune remodeling that allows virus production. The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) papain-like protease (PLpro) encoded in the large nonstructural protein 3 (Nsp3) also possesses isopeptidase activity with specificity for ubiquitin and ISG15 conjugates. Here, we interrogated the cellular interactome of the SARS-CoV-2 PLpro catalytic domain to gain insight into the putative substrates and cellular functions affected by the viral deubiquitinase.
View Article and Find Full Text PDFIdentification of a potential anti-viral drug targeting allosteric site of papain-like protease against rubella using a molecular modeling approach.
J Biomol Struct Dyn
December 2024
Department of Biological Sciences, Sunandan Divatia School of Science, SVKM's Narsee Monjee Institute of Management Studies (NMIMS) Deemed-to-be University, Mumbai, Maharashtra, India.
Rubella virus (RUBV) is responsible for causing rashes, lymphadenopathy, and fever which are the hallmarks of an acute viral illness called Rubella. For RUBV replication, the non-structural polyprotein p200 must be cleaved by the rubella papain-like protease (RubPro) into the multifunctional proteins p150 and p90. Hence, RubPro is an attractive target for anti-viral drug discovery.
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