Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0039-128x(69)80024-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A simple automated assay format for measuring multiple immune checkpoint inhibitors.
J Pharm Biomed Anal
December 2024
Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway.
Immune checkpoint inhibitors (ICIs) have improved survival rates in oncology, but there is a rising concern for immune-related adverse health outcomes. Monitoring drug serum concentration would enable tailored dosing, however this strategy has not yet been evaluated for ICIs. Fully automated analyte capture assays with time-resolved fluorometry using protein A as tracer, were developed for three different ICIs; the cytotoxic T lymphocyte Antigen-4 (CTLA4) inhibitor ipilimumab (Yervoy; Bristol-Myers Squibb) and the Programmed Death-1 (PD-1) inhibitors nivolumab (Opdivo; Bristol-Myers Squibb) and pembrolizumab (Keytruda; Merck).
View Article and Find Full Text PDFSupervised multi-frame dual-channel denoising enables long-term single-molecule FRET under extremely low photon budget.
Nat Commun
January 2025
State Key Laboratory of Membrane Biology, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing, China.
Camera-based single-molecule techniques have emerged as crucial tools in revolutionizing the understanding of biochemical and cellular processes due to their ability to capture dynamic processes with high precision, high-throughput capabilities, and methodological maturity. However, the stringent requirement in photon number per frame and the limited number of photons emitted by each fluorophore before photobleaching pose a challenge to achieving both high temporal resolution and long observation times. In this work, we introduce MUFFLE, a supervised deep-learning denoising method that enables single-molecule FRET with up to 10-fold reduction in photon requirement per frame.
View Article and Find Full Text PDFNeuromorphic-enabled video-activated cell sorting.
Nat Commun
December 2024
State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing, China.
Imaging flow cytometry allows image-activated cell sorting (IACS) with enhanced feature dimensions in cellular morphology, structure, and composition. However, existing IACS frameworks suffer from the challenges of 3D information loss and processing latency dilemma in real-time sorting operation. Herein, we establish a neuromorphic-enabled video-activated cell sorter (NEVACS) framework, designed to achieve high-dimensional spatiotemporal characterization content alongside high-throughput sorting of particles in wide field of view.
View Article and Find Full Text PDFAn integrated microflow cytometry platform with artificial intelligence capabilities for point-of-care cellular phenotype analysis.
Biosens Bioelectron
March 2025
Department of Biotechnology, National Formosa University, No. 64, Wunhua Rd, Huwei Township, Yunlin County, 63201, Taiwan. Electronic address:
The EZ DEVICE is an integrated fluorescence microflow cytometer designed for automated cell phenotyping and enumeration using artificial intelligence (AI). The platform consists of a laser diode, optical filter, objective lens, CMOS image sensor, and microfluidic chip, enabling automated sample pretreatment, labeling, and detection within a single compact unit. AI algorithms segment and identify objects in images captured by the CMOS sensor at 532 and 586 nm emission wavelengths.
View Article and Find Full Text PDFMethods for Visualizing and Quantifying Cholesterol Distribution in Mammalian Cells Using Filipin and D4 Probes.
Methods Mol Biol
December 2024
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), UMR 7104, CNRS, Inserm, Université de Strasbourg, Illkirch, France.
Cholesterol is a key component of biological membranes and, like many cellular lipids, is unevenly distributed among organelles. Disruptions in cholesterol trafficking are associated with various pathologies, including lysosomal lipid storage disorders, often characterized by intracellular cholesterol accumulation. A significant challenge in studying cholesterol trafficking is the lack of easy methods to trace this molecule in situ.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!