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Redesigning methionine γ-lyase for improved stability and catalytic activity in the β-elimination reaction for the synthesis of thiosulfinates.
Biochimie
January 2025
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences, Vavilov street, 32, Moscow, 119991, Russia.
Pyridoxal 5'-phosphate (PLP)-dependent enzymes are involved in many cellular processes and possess unequalled catalytic versatility. Rational design through site-directed mutagenesis is a powerful strategy for creating tailor-made enzymes for a wide range of biocatalytic applications. PLP-dependent methionine γ-lyase (MGL), which degrades sulfur-containing amino acids, is an encouraging enzyme for many therapeutic purposes - from combating bacterial resistant strains and fungi to antitumor activity.
View Article and Find Full Text PDFViral auxiliary roles in hydrolytic and biosynthetic metabolism regulate prokaryotic microbial interactions in anaerobic digestion.
Water Res
January 2025
MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou, 310058, China. Electronic address:
Anaerobic digestion (AD) viruses have gained recognition as significant regulators of microbial interactions within AD communities, yet their ecological roles remain largely unexplored. In this study, we investigated the ecological roles of AD viruses in regulating microbial interactions among syntrophic hosts. We recovered 3921 diverse viral sequences from four full-scale anaerobic digesters and confirmed their widespread presence across 127 global metagenomic sampling sites (with >95 % sequence similarity), underscoring the ubiquity of prokaryotic viruses in AD-related systems.
View Article and Find Full Text PDFDiscovery of proteolytically stable monocyte locomotion inhibitory factor peptide through systematic structural optimization.
Eur J Med Chem
January 2025
Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China; Shanghai Integration and Innovation Center of Marine Medical Engineering, China. Electronic address:
The identification of novel molecular candidates capable of treating osteoarthritis (OA) has significant clinical implications. Monocyte locomotion inhibitory factor peptide (MLIF) is a pentapeptide derived from Entamoeba histolytica. It has been found possesses selective anti-inflammatory effects both in vitro and in vivo.
View Article and Find Full Text PDFCreatine monohydrate administration delayed muscle glycolysis of antemortem-stressed broilers by enhancing muscle energy status, increasing antioxidant capacity and regulating muscle metabolite profiles.
Poult Sci
January 2025
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266000, China; Department of Biology and Agriculture, Zunyi Normal College, Ping`an Avenue, Hong Huagang District, Zunyi 563006, China.
Preslaughter stress induced a negative energy balance of broilers, resulted in an accelerated glycolysis and finally led to an inferior meat quality. The present study aimed to investigate the effects of creatine monohydrate (CMH) supplementation on muscle energy storage, antioxidant capacity, the glycolysis of postmortem muscle and the metabolite profiles in muscle of broilers subjected to preslaughter transport. Two hundred and forty broilers were chosen and randomly allocated into three treatments (group A, group B and group C), comprising 8 replicates (10 broilers each replicate).
View Article and Find Full Text PDFNitrogen@Carbon Quantum Dots for Fluorescence Detection of L-alanine, L-methionine and L-cysteine.
J Fluoresc
January 2025
School of Materials and Chemical Engineering, West Anhui University, Lu'an, Anhui, 237012, China.
Nitrogen@Carbon quantum dots (N@CQDs) are prepared using microwave hydrothermal method, and polyvinylpyrrolidone (PVP) and melamine are used as mixed C source and N source. Microwave reaction conditions of preparing the N@CQDs are 170 ℃ and 3 h. This N@CQDs are are used as fluorescence probe for detection of amino acids.
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