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A high-throughput ResNet CNN approach for automated grapevine leaf hair quantification.
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January 2025
Julius Kühn Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Grapevine Breeding Geilweilerhof, 76833, Siebeldingen, Germany.
The hairiness of the leaves is an essential morphological feature within the genus Vitis that can serve as a physical barrier. A high leaf hair density present on the abaxial surface of the grapevine leaves influences their wettability by repelling forces, thus preventing pathogen attack such as downy mildew and anthracnose. Moreover, leaf hairs as a favorable habitat may considerably affect the abundance of biological control agents.
View Article and Find Full Text PDFAirborne Acoustic Vortex End Effector-based Contactless, Multi-mode, Programmable Control of Object Surfing.
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September 2024
Fralin Biomedical Research Institute, Virginia Polytechnic Institute and State University, Roanoke, VA, 24016, USA.
Tweezers based on optical, electric, magnetic, and acoustic fields have shown great potential for contactless object manipulation. However, current tweezers designed for manipulating millimeter-sized objects such as droplets, particles, and small animals, exhibit limitations in translation resolution, range, and path complexity. Here, we introduce a novel acoustic vortex tweezers system, which leverages a unique airborne acoustic vortex end effector integrated with a three degree-of-freedom (DoF) linear motion stage, for enabling contactless, multi-mode, programmable manipulation of millimeter-sized objects.
View Article and Find Full Text PDFFlow-on-repellent biofabrication of fibrous decellularized breast tumor-stroma models.
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Department of Chemistry, CICECO - Aveiro Institute of Materials, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal. Electronic address:
On-the-fly biofabrication of reproducible 3D tumor models at a pre-clinical level is highly desirable to level-up their applicability and predictive potential. Incorporating ECM biomolecular cues and its complex 3D bioarchitecture in the design stages of such in vitro platforms is essential to better recapitulate the native tumor microenvironment. To materialize these needs, herein we describe an innovative flow-on-repellent (FLORE) 3D extrusion bioprinting technique that leverages expedited and automatized bioink deposition onto a customized superhydrophobic printing bed.
View Article and Find Full Text PDFCharacterization of AI-enhanced magnetophoretic transistors operating in a tri-axial magnetic field for on-chip bioparticle sorting.
Sci Rep
October 2024
Faculty of Mechanical Engineering, Tarbiat Modares University, P.O. Box: 14115-111, Tehran, Iran.
We demonstrate two general classes of magnetophoretic transistors, called the "trap" and the "repel-and-collect" transistors, capable of switching single magnetically labeled cells and magnetic particles between different paths in a microfluidic chamber. Compared with prior work on magnetophoretic transistors operating in a two-dimensional in-plane rotating field, the use of a tri-axial magnetic field has the fundamental advantages of preventing particle cluster formation and better syncing of single particles with the general operating clock. We use finite element methods to investigate the energy distribution on the chip surface and to predict the particle behavior at various device geometries.
View Article and Find Full Text PDFAntithrombogenic polysaccharide coatings to improve hemocompatibility, protein-repellence, and endothelial cell response.
iScience
September 2024
University of Maribor, Faculty of Mechanical Engineering, Laboratory for Characterisation and Processing of Polymers, Smetanova ulica17, 2000 Maribor, Slovenia.
Polyester biomaterials play a crucial in vascular surgery, but suffer from unspecific protein adsorption, thrombogenicity, and inadequate endothelial cell response, which limit their success. To address these issues, we investigated the functionalization of polyester biomaterials with antithrombogenic polysaccharide coatings. A two-step and water-based method was used to coat cationized polycaprolactone with different sulfated polysaccharides (SPS), which resulted in long-term stability, tunable morphology, roughness, film thickness, chemical compositions, zeta potential, and water content.
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