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Rapid Tryptophan Assay as a Screening Procedure for Quality Protein Maize.
Molecules
September 2024
Integrated Centre of Environmental Science Studies in the North East Region-CERNESIM Centre, Institute of Interdisciplinary Research, Alexandru Ioan Cuza University of Iasi, 11 Carol I, 700506 Iasi, Romania.
Tryptophan is an essential amino acid deficient in cereals, especially maize. However, maize ( L.) is the main source of protein in some developing countries in Africa and Latin America.
View Article and Find Full Text PDFEstimation of gingival crevicular fluid oxidative stress markers in school-aged children and teenagers with insufficient sleep.
BMC Oral Health
December 2022
Department of Cariology and Endodontics, Nanjing Stomatological Hospital, Medical School, Nanjing University, No. 30, Central Road, Nanjing, 210000, People's Republic of China.
Background: Sleep is crucial for survival. Sleep deprivation causes ROS accumulation and, consequently, oxidative stress. The goal of the study was to evaluate gingival crevicular fluid (GCF) levels of the oxidative stress status hydrogen peroxide (HO), superoxide glutathione (GSH), and cellular oxidative damage marker malondialdehyde (MDA) in school-aged children and teenagers with insufficient sleep.
View Article and Find Full Text PDFTotal protein quantitation using the bicinchoninic acid assay and gradient elution moving boundary electrophoresis.
Electrophoresis
July 2014
National Institute of Standards and Technology, Gaithersburg, MD, USA.
We investigated the ability of gradient elution moving boundary electrophoresis (GEMBE) with capacitively coupled contactless conductivity detection (C(4) D) to assay total protein concentration using the bicinchoninic acid (BCA) reaction. We chose this format because GEMBE-C(4) D behaves as a concentration dependent detection system, unlike optical methods that also rely on pathlength (due to Beer's law). This system tolerates proteins well compared with other capillary electrophoretic methods, allowing the capillary to be reused without coatings or additional hydroxide wash steps.
View Article and Find Full Text PDFIn vitro neutralization of viral hemorrhagic septicemia virus by plasma from immunized zebrafish.
Zebrafish
March 2013
Dpto. Biotecnología, Instituto Nacional Investigaciones Agrarias, Madrid, Spain.
We studied humoral long-term adaptive viral neutralization responses in zebrafish (Danio rerio), an increasingly useful vertebrate model for viral diseases actually limited by the absence of standardized anti-zebrafish immunoglobulin M (IgM) antibodies. We established an alternative method, similar to those used in other fish, to achieve a first estimation of zebrafish anti-viral antibody-like responses. We used the viral hemorrhagic septicemia virus (VHSV) model because, although protection after this non-natural infection was demonstrated in cold-acclimatized zebrafish, little is known about their induced anti-VHSV antibody-like responses.
View Article and Find Full Text PDFComparative evaluation of two quantitative test methods for determining the efficacy of liquid sporicides and sterilants on a hard surface: a precollaborative study.
J AOAC Int
July 2007
U.S. Environmental Protection Agency-Office of Pesticide Programs, Microbiology Laboratory, Environmental Science Center, Ft. Meade, MD 20755-5350, USA.
Two quantitative carrier-based test methods for determining the efficacy of liquid sporicides and sterilants on a hard surface, the Standard Quantitative Carrier Test Method-ASTM E 2111-00 and an adaptation of a quantitative micro-method as reported by Sagripanti and Bonifacino, were compared in this study. The methods were selected based on their desirable characteristics (e.g.
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